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  4. Recombinant Enterobacteria phage P21 Terminase small subunit (1)

Recombinant Enterobacteria phage P21 Terminase small subunit (1)

  • 货号：
    CSB-YP301580EDF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP301580EDF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP301580EDF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP301580EDF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP301580EDF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    1
  • Uniprot No.：
  • 别名：
    1; nohA; Terminase; small subunit; Gp1
  • 种属：
    Enterobacteria phage P21 (Bacteriophage 21) (Bacteriophage P21)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-182
  • 氨基酸序列
    MKVNKKRLAE IFNVDPRTIE RWQSQGLPCA SKGSKGIESV FDTAMAIQWY AQRETDIENE KLRKELDDLR AAAESDLQPG TIDYERYRLT KAQADAQELK NAREDGVVLE TELFTFILQR VAQEISGILV RVPLTLQRKY PDISPSHLDV VKTEIAKASN VAAKAGENVG GWIDDFRRAE GS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the terminase that processes and encapsidates viral genomes during virion assembly. The terminase is composed of two small and one large subunits. To initiate packaging, it binds a specific sequence, at the junction of adjacent viral genomes in the concatemeric DNA substrate. Next, in a reaction stimulated by the presence of proheads and ATP but not requiring ATP hydrolysis, the terminase creates two nicks at a specific site, one on each stand. Terminase then separates the cohesive ends in a reaction requiring ATP hydrolysis. The heterotrimer remains bound to the left end of the genome to be packaged, forming a stable DNA-protein complex known as complex I. In a reaction facilitated by a viral assembly catalyst, gpFI, complex I binds a prohead, a preformed head shell precursor, to form complex II. In another packaging reaction requiring ATP hydrolysis, the DNA is translocated into the prohead until the next specific site on the concatemer reaches the packaging complex. At this time the downstream specific site is cut and the heterotrimer undocks from the DNA-filled head to remain bound to the left end of concatemer's next genome. The new heterotrimer-DNA complex I binds another prohead to continue the processive, polarized packaging of viral genomes. The terminase is dependent upon host integration host factor (ihfA/ihfB) for these activities.
  • 亚细胞定位：
    Host cytoplasm.
  • 蛋白家族：
    Terminase small subunit family