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  4. Recombinant Enterobacteria phage T7 Bacterial RNA polymerase inhibitor (2)

Recombinant Enterobacteria phage T7 Bacterial RNA polymerase inhibitor (2)

  • 货号：
    CSB-YP356123EEB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356123EEB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356123EEB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356123EEB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356123EEB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    2
  • Uniprot No.：
  • 别名：
    2; Bacterial RNA polymerase inhibitor; Gene product 2; Gp2
  • 种属：
    Enterobacteria phage T7 (Bacteriophage T7)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-64
  • 氨基酸序列
    MSNVNTGSLS VDNKKFWATV ESSEHSFEVP IYAETLDEAL ELAEWQYVPA GFEVTRVRPC VAPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as an inhibitor of bacterial RNA polymerase by interacting with RpoC subunit and thus preventing promoter recognition by RpoE. Non-DNA-binding transcription factor that plays a role in the transcriptional switch that abolishes transcription of the viral early genes (class I) by the host RNA polymerase but allows transcription of late genes (class II and class III) by T7 RNA polymerase.
  • 基因功能参考文献：
    1. Overexpression of recombinant inhibitor of host bacterial RNA polymerase (Gp2) in exponentially growing E. coli induces changes in RNAP behavior at single molecule level and induces global alterations in the transcriptional programme. PMID: 28977482
    2. gp15 and gp16 can refold from a partially denatured state in vitro, and that gp15 interacts with gp16 to form a spiral ring structure. PMID: 26476287
    3. E24K and F27Y mutations of Gp2 facilitate the interaction between Gp2beta and Escherichia coli RNA polymerase. PMID: 22977089
    4. Gp2 inhibits RPo formation not just by steric occlusion of the RNA polymerase (RNAp)-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of the amino-terminal sigma(70) domain region 1.1 (R1.1) by Gp2. PMID: 22819324
    5. comprehensive mutagenesis data reveal that two conserved arginine residues located on the opposite side of Gp2 are important for binding to and inhibition of RNAP PMID: 20133868

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  • 数据库链接：

    KEGG: vg:1261073