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  4. Recombinant Escherichia coli ABC transporter ATP-binding protein uup (uup)

Recombinant Escherichia coli ABC transporter ATP-binding protein uup (uup)

  • 中文名称：
    大肠杆菌uup重组蛋白
  • 货号：
    CSB-YP337484ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌uup重组蛋白
  • 货号：
    CSB-EP337484ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌uup重组蛋白
  • 货号：
    CSB-EP337484ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌uup重组蛋白
  • 货号：
    CSB-BP337484ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌uup重组蛋白
  • 货号：
    CSB-MP337484ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    uup
  • Uniprot No.：
  • 别名：
    uup; ycbH; ycbI; b0949; JW0932; ATP-binding protein Uup; EC 3.6.1.3
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-635
  • 氨基酸序列
    MSLISMHGAW LSFSDAPLLD NAELHIEDNE RVCLVGRNGA GKSTLMKILN REQGLDDGRI IYEQDLIVAR LQQDPPRNVE GSVYDFVAEG IEEQAEYLKR YHDISRLVMN DPSEKNLNEL AKVQEQLDHH NLWQLENRIN EVLAQLGLDP NVALSSLSGG WLRKAALGRA LVSNPRVLLL DEPTNHLDIE TIDWLEGFLK TFNGTIIFIS HDRSFIRNMA TRIVDLDRGK LVTYPGNYDQ YLLEKEEALR VEELQNAEFD RKLAQEEVWI RQGIKARRTR NEGRVRALKA MRRERGERRE VMGTAKMQVE EASRSGKIVF EMEDVCYQVN GKQLVKDFSA QVLRGDKIAL IGPNGCGKTT LLKLMLGQLQ ADSGRIHVGT KLEVAYFDQH RAELDPDKTV MDNLAEGKQE VMVNGKPRHV LGYLQDFLFH PKRAMTPVRA LSGGERNRLL LARLFLKPSN LLILDEPTND LDVETLELLE ELIDSYQGTV LLVSHDRQFV DNTVTECWIF EGGGKIGRYV GGYHDARGQQ EQYVALKQPA VKKTEEAAAA KAETVKRSSS KLSYKLQREL EQLPQLLEDL EAKLEALQTQ VADASFFSQP HEQTQKVLAD MAAAEQELEQ AFERWEYLEA LKNGG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably plays a role in ribosome assembly or function; overexpression suppresses cold-sensitive growth of a bipA deletion (Ref.10). May be involved in resolution of branched DNA intermediates that result from template switching in postreplication gaps. Binds DNA at Holliday junctions. May be involved in the correct segregation of nucleoids. Has ATPase activity, binds DNA non-sequence specifically; the presence of DNA does not change the ATPase activity. Mutations in this gene cause an increase in RecA-independent precise excision of transposons and insertion elements, and also reduce bacteriophage Mu growth.
  • 基因功能参考文献：
    1. compared the DNA binding activities of three versions of the protein (Uup, UupDeltaCTD and CTD) by an electrophoretic mobility shift assay. CTD is able to bind DNA although less efficiently than intact Uup and UupDeltaCTD (C-terminal domain). PMID: 19948254
    2. ATP hydrolysis at the two ATP-binding cassette domains is strictly coordinated and is essential for the cytosolic ATP-binding cassette (ABC) ATPase function of Uup in vivo. PMID: 16407313
    3. Deletion of the uup gene, which encodes a soluble ATP binding cassette (ABC) ATPase, renders the mutant strain sensitive to its parent when they are grown together in the same medium. PMID: 18848624
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    ABC transporter superfamily, ABCF family, EF3 subfamily
  • 数据库链接：

    KEGG: ecj:JW0932

    STRING: 316385.ECDH10B_1019