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  4. Recombinant Escherichia coli ATP-dependent DNA helicase rep (rep)

Recombinant Escherichia coli ATP-dependent DNA helicase rep (rep)

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  • 中文名称：
    大肠杆菌rep重组蛋白
  • 货号：
    CSB-RP095644Ba
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    rep
  • Uniprot No.：
  • 别名：
    rep; b3778; JW5604; ATP-dependent DNA helicase Rep; EC 3.6.4.12
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    104.0kDa
  • 表达区域：
    1-673aa
  • 氨基酸序列
    MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGYEALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFPYVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRPEPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this Recombinant E.coli K12 MPL protein began at the genetic level, where the coding sequence for the MPL protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (E.coli) so that the cells could be cultured and expressed the desired rep protein. And we finally got the recombinant rep protein with the purity of 90%+ determined by SDS-PAGE.

    Extracellular adenosine triphosphate (ATPe) is an important signaling molecule, which regulates a wide range of cellular functions including cell proliferation, apoptosis, immune response, neurotransmission and, focus of our interest, the epithelial ion transport. In order to achieve these effects, ATP is released from cells in response to a number of stimuli including transmitter or hormone stimulation, shear stress, cell volume changes, hypoxia and release from damaged cells. Several ATP release mechanisms have been proposed to explain transport of ATP across the plasma membrane. One of the ATP releasing pathways is the regulated vesicular exocytosis, which occurs in neurons, endocrine cells and some exocrine cells, and constitutive vesicular transport. Other models of ATP release pathways include anion channels and transporters (e.g. maxi-anion channels, volumeregulated anion channels, cystic fibrosis transmembrane conductance regulator (CFTR)), multidrug resistance proteins, pannexin-1 and pannexin-1 in conjunction with the P2X7 receptor and connexin hemichannels.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.
  • 基因功能参考文献：
    1. After replication blockage by an inverted rrn, Rep in conjunction with DinG or UvrD removes RNA polymerase, a task that is fulfilled in its absence by the SOS-induced DinG and UvrD helicases. PMID: 19851282
    2. Rep helicase activity is regulated by the rotational conformational state of its 2B subdomain; formation of a Rep dimer may relieve autoinhibition by altering the 2B subdomain orientation. PMID: 16009938
    3. We examined the effect of a rep mutation on illegitimate recombination and found that the frequency of spontaneous illegitimate recombination is enhanced by the rep mutation PMID: 16236131
    4. the binding of a single Rep monomer to a stalled replication fork can lead to repetitive shuttling along the single-stranded region, possibly keeping the DNA clear of toxic recombination intermediates PMID: 16251956
  • 蛋白家族：
    Helicase family, UvrD subfamily
  • 数据库链接：

    KEGG: ecj:JW5604

    STRING: 316385.ECDH10B_3967