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  4. Recombinant Escherichia coli ATP synthase gamma chain (atpG)

Recombinant Escherichia coli ATP synthase gamma chain (atpG)

  • 中文名称：
    Recombinant Escherichia coli ATP synthase gamma chain(atpG),Yeast
  • 货号：
    CSB-YP364627ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP synthase gamma chain(atpG),Yeast
  • 货号：
    CSB-EP364627ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP synthase gamma chain(atpG),Yeast
  • 货号：
    CSB-EP364627ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP synthase gamma chain(atpG),Yeast
  • 货号：
    CSB-BP364627ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli ATP synthase gamma chain(atpG),Yeast
  • 货号：
    CSB-MP364627ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    atpG
  • Uniprot No.：
  • 别名：
    atpG; papC; uncG; b3733; JW3711ATP synthase gamma chain; ATP synthase F1 sector gamma subunit; F-ATPase gamma subunit
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-287
  • 氨基酸序列
    MAGAKEIRSK IASVQNTQKI TKAMEMVAAS KMRKSQDRMA ASRPYAETMR KVIGHLAHGN LEYKHPYLED RDVKRVGYLV VSTDRGLCGG LNINLFKKLL AEMKTWTDKG VQCDLAMIGS KGVSFFNSVG GNVVAQVTGM GDNPSLSELI GPVKVMLQAY DEGRLDKLYI VSNKFINTMS QVPTISQLLP LPASDDDDLK HKSWDYLYEP DPKALLDTLL RRYVESQVYQ GVVENLASEQ AARMVAMKAA TDNGGSLIKE LQLVYNKARQ ASITQELTEI VSGAAAV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.
  • 基因功能参考文献：
    1. A series of angular accelerations and decelerations occur during the power stroke of the F!-ATPase molecular motor. PMID: 24567403
    2. The catalytic activity of PapC is required to initiate pilus biogenesis. PMID: 21299650
    3. analysis of epsilon and gamma subunits roles in inhibited and active states of Escherichia coli ATP synthase F1 rotation PMID: 20974856
    4. The PapC usher domain structure reveals a beta-sandwich fold very similar to that of the plug domain, a domain of PapC obstructing its translocation domain. PMID: 20118254
    5. PapC topology analysis by site-directed fluorescence labeling PMID: 15485883
    6. overall molecular size (11 nm), pore size (2 nm), and twin-pore configuration of PapC resemble that of the Tom40 complex, a mitochondrial outer membrane protein translocase PMID: 15561151
    7. PapC and FimD of Uropathogenic E coli are ushers bound to a translocating type 1 pilus assembly intermediate; they capture the secretion of a virulence factor across the outer membrane. PMID: 18485872
    8. Prevalence of P fimbriae (papC), capsule K5 (sfaD/E) and haemolysin (hlyA) genes was higher in E. coli isolated from active and non-active coeliac children than in those from control subjects [papC; sfaD/E; hlyA]. PMID: 18983674
    9. the temperature dependence of single molecule rotation of the Escherichia coli ATP synthase F1 sector reveals the importance of gamma-beta subunit interactions in the catalytic dwell PMID: 19502237

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    ATPase gamma chain family
  • 数据库链接：

    KEGG: ecj:JW3711

    STRING: 316385.ECDH10B_3920