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  4. Recombinant Escherichia coli Beta-lactamase (ampC)

Recombinant Escherichia coli Beta-lactamase (ampC)

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  • 中文名称：
    Recombinant Escherichia coli Beta-lactamase(ampC)
  • 货号：
    CSB-EP360448ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    ampC
  • Uniprot No.：
  • 别名：
    ampC; ampA; b4150; JW4111Beta-lactamase; EC 3.5.2.6; Cephalosporinase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Mature full length protein
  • 来源：
    E.coli
  • 分子量：
    52.5 kDa
  • 表达区域：
    20-377aa
  • 氨基酸序列
    APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Just like other recombinant proteins, the production of this recombinant E.coli ampC protein began with appropriate cDNA and PCR methods, and then the ampC expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant E.coli ampC protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 85%+.

    ampC (also called ampA) is a gene providing an instruction of making a protein named beta-lactamase (also known as cephalosporinase) in Escherichia coli (E.coli). This protein belongs to Class-C beta-lactamase family, which are enzymes produced by bacteria, E.coli and Klebsiella. The protein encoded by ampC hydrolyzes the beta-lactam bond in susceptible beta-lactam antibiotics and takes part in antibiotic catabolic process and the process of response to antibiotic.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
  • 基因功能参考文献：
    1. These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli. PMID: 26989692
    2. Recently, high-level AmpC production has also been seen in new species such as Escherichia coli via plasmid acquisition. PMID: 25643271
    3. the majority of the strains of E. coli with AmpC-susceptibility pattern are pAmpC positive, although the association of extended-spectrum beta-lactamases (ESBL) and pAmpC is unusual. PMID: 24372614
    4. A high prevalence of birds carries extended spectrumBL(ESBL)/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. PMID: 22949623
    5. Extended-spectrum beta-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. PMID: 23243181
    6. Cefoxitin resistance in E. coli N99-0001 was due to overexpression of ampC caused by an increase in promoter strength. PMID: 15761065
    7. determined structure of ampC K67R mutant by X-ray crystallography in apo & transition state-analog complexed forms; results suggest arginine side chain is unable to play the same catalytic role as Lys67 in either the acylation or deacylation reactions PMID: 19241376

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  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    Class-C beta-lactamase family
  • 数据库链接：

    KEGG: ecj:JW4111

    STRING: 316385.ECDH10B_4345