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  4. Recombinant Escherichia coli Bifunctional protein BirA (birA)

Recombinant Escherichia coli Bifunctional protein BirA (birA)

  • 货号：
    CSB-YP361925ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP361925ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP361925ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP361925ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP361925ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    birA
  • Uniprot No.：
  • 别名：
    birA; bioR; dhbB; b3973; JW3941; Bifunctional ligase/repressor BirA; Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] ligase; EC 6.3.4.15; Biotin--protein ligase; Biotin-[acetyl-CoA carboxylase] synthetase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-321
  • 氨基酸序列
    MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
  • 基因功能参考文献：
    1. BirA signals to the global regulator Fur, which in turn activates LEE (locus of enterocyte effacement) genes to promote EHEC adherence in the low-biotin large intestine. PMID: 25791315
    2. work demonstrates that variable surface loops evolved to support BirA function in the homodimerization required for transcription repression by complementing the constant loops, which are constrained because of their essential role in posttranslational biotin addition PMID: 22446587
    3. BirA function entails extensive interactions among the three domains of the protein and show that normal ligase activity does not ensure normal DNA binding. PMID: 22210766
    4. Results describe the 2.8A resolution crystal structure of BirA in complex with the co-repressor analog biotinol-5'-AMP. PMID: 16438984
    5. Results indicate that the hydrophobic cluster is critical to the ligand-induced disorder-to-order transition in birA and that this transition is integral to the allosteric response in the biotin repressor. PMID: 17765263
    6. BirA ligase biotinylates K4, K9, K18 and K23 in histone H3. PMID: 18452652
    7. energetic and structural underpinnings of the dual specificity of protein BirA as a homodimer or as a heterodimer with acetyl coenzyme A carboxylase PMID: 19361526

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  • 蛋白家族：
    Biotin--protein ligase family
  • 数据库链接：

    KEGG: ecj:JW3941

    STRING: 316385.ECDH10B_4162