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  4. Recombinant Escherichia coli Colicin-E1 (cea), partial

Recombinant Escherichia coli Colicin-E1 (cea), partial

In Stock
  • 货号：
    CSB-EP360926ENL1
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    cea
  • Uniprot No.：
  • 别名：
    cea; Colicin-E1
  • 种属：
    Escherichia coli
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    38.2 kDa
  • 表达区域：
    1-304aa
  • 氨基酸序列
    METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant Escherichia coli cea protein is an E.coli-expressed partial protein. Molecularly, this cea protein is characterized by N-terminal 10xHis tag and C-terminal Myc tag, internal Escherichia coli cea DNA fragment (1-304aa). Standard methods for recombinant cea protein expression comprise transfecting cells with DNA vectors that consist of specific templates and then cultures cells to translate and transcribe the cea protein production process. Typically, these cells are lysed to extract expressed proteins for more purification. The purity of this recombinant cea protein is 85%+ measured by SDS-PAGE.

    cea, also known as colicin E1, is a bacteriocin produced by E. coli that acts against bacteria by forming a pore in the bacterial membrane, leading to membrane depolarization and cell death. The cobalamin translocator BtuB binds to the colicin E1, initiating the import of colicin E1. The drug-export protein TolC is essential for the import of colicin E1 across the outer membrane and periplasmic space. Treatment of cells harboring the plasmid ColE1 with chemicals that damage DNA or interfere with DNA synthesis can result in the production of colicin E1.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.; Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Channel forming colicin family