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  4. Recombinant Escherichia coli CsgBAC operon transcriptional regulatory protein (csgD)

Recombinant Escherichia coli CsgBAC operon transcriptional regulatory protein (csgD)

  • 货号：
    CSB-YP344759ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP344759ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP344759ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP344759ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    csgD
  • Uniprot No.：
  • 别名：
    csgD; b1040; JW1023CsgBAC operon transcriptional regulatory protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-216
  • 氨基酸序列
    MFNEVHSIHG HTLLLITKSS LQATALLQHL KQSLAITGKL HNIQRSLDDI SSGSIILLDM MEADKKLIHY WQDTLSRKNN NIKILLLNTP EDYPYRDIEN WPHINGVFYS MEDQERVVNG LQGVLRGECY FTQKLASYLI THSGNYRYNS TESALLTHRE KEILNKLRIG ASNNEIARSL FISENTVKTH LYNLFKKIAV KNRTQAVSWA NDNLRR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription. Low-level constitutive expression confers an adherent curli fimbriae-expressing phenotype, up-regulates 10 genes and down-regulates 14 others.
  • 基因功能参考文献：
    1. Regulation of the master biofilm regulator, CsgD, was diverse, and the screen revealed several proteins and small RNAs (sRNA) that regulate csgD messenger RNA (mRNA) levels. Using previously published studies, we found minimal overlap between the genes affecting curli biogenesis and genes known to impact swimming or swarming motility, underlying the distinction between motile and sessile lifestyles. PMID: 29088115
    2. Promoter mutations in csgD that alter sigma factor utilization from RpoS to RpoD provide a unique mechanism for increasing biofilm formation, but the transition also disrupts normal rpoS-dependent CsgD regulation. PMID: 28406080
    3. RprA can directly reduce csgD mRNA levels and translation, and ce transcription of csgD. PMID: 22356413
    4. The s propose that the cell motility for planktonic growth is repressed by CsgD, thereby promoting the switch to biofilm formation. PMID: 21421764
    5. Five different transcription factors interact to regulate the CsgD promoter. PMID: 20466769
    6. CsgD activation of glyA requires the participation of both known regulatory proteins, MetR and PurR PMID: 15644921
    7. role of the interplay OmpR and CpxR in affecting the transcription of the curli gene in response to variation of the medium osmolarity; CpxR represses csgD expression in high-salt-content medium, resulting in low curli production PMID: 15743952
    8. Our results indicate that CsgD can modulate cellulose biosynthesis through activation of the yoaD gene. In addition, the positive effect of CsgD on biofilm formation might be enhanced by repression of the fecR gene. PMID: 16513732

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein. Note=In experiments done with low-level constitutively expressed protein.
  • 数据库链接：

    KEGG: ecj:JW1023

    STRING: 316385.ECDH10B_1112