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  4. Recombinant Escherichia coli DNA-binding protein stpA (stpA)

Recombinant Escherichia coli DNA-binding protein stpA (stpA)

  • 中文名称：
    大肠杆菌stpA重组蛋白
  • 货号：
    CSB-YP364796ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌stpA重组蛋白
  • 货号：
    CSB-EP364796ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌stpA重组蛋白
  • 货号：
    CSB-EP364796ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌stpA重组蛋白
  • 货号：
    CSB-BP364796ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌stpA重组蛋白
  • 货号：
    CSB-MP364796ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    stpA
  • Uniprot No.：
  • 别名：
    stpA; hnsB; b2669; JW2644; DNA-binding protein StpA; H-NS homolog StpA
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-134
  • 氨基酸序列
    MSVMLQSLNN IRTLRAMARE FSIDVLEEML EKFRVVTKER REEEEQQQRE LAERQEKIST WLELMKADGI NPEELLGNSS AAAPRAGKKR QPRPAKYKFT DVNGETKTWT GQGRTPKPIA QALAEGKSLD DFLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A DNA-binding protein that acts in a fashion similar to H-NS protein upon overexpression, represses a number of genes including the cryptic blg operon, hns, papB and the proU locus. A subset of H-NS/StpA-regulated genes also require Hha for repression; Hha and Cnu (YdgT) increases the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex. Repression can be inhibited by dominant-negative mutants of StpA or H-NS.; (Microbial infection) Originally isolated as a suppressor of a splicing defect of the thymidylate synthase (td) gene from bacteriophage T4. Acts as an RNA chaperone, accelerating splicing of viral pre-mRNA. Binds preferentially to unstructured over structured RNA; does not have a detectable high-affinity RNA-binding site in the pre-mRNA. There do not seem to be any specific RNA targets in transcribed E.coli DNA.
  • 基因功能参考文献：
    1. In contrast to H-NS, the StpA filament interacts with a naked DNA segment to cause DNA bridging which results in simultaneous stiffening and bridging of DNA. PMID: 22187157
    2. forms complexes with YdgT in vivo PMID: 15458420
    3. The structural stability of an RNA determines whether the RNA chaperone activity of StpA is beneficial to folding. PMID: 15849314
    4. StpA binding hinders DNA cleavage by both the non-specific endonuclease, DNase I, and by the site-specific type I restriction endonuclease, EcoKI. PMID: 16299353
    5. Analysis of the relationship of stpA RNA chaperone activity to its RNA-binding properties. PMID: 17267410
    6. StpA in homologous recombination and double-strand break repair is discussed PMID: 17991999

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  • 亚细胞定位：
    Cytoplasm, nucleoid.
  • 蛋白家族：
    Histone-like protein H-NS family
  • 数据库链接：

    KEGG: ecj:JW2644

    STRING: 316385.ECDH10B_2836