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  4. Recombinant Escherichia coli DNA helicase II (uvrD)

Recombinant Escherichia coli DNA helicase II (uvrD)

  • 货号：
    CSB-YP360936ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP360936ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP360936ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP360936ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    uvrD
  • Uniprot No.：
  • 别名：
    uvrD; mutU; pdeB; rad; recL; b3813; JW3786; DNA helicase II; EC 3.6.4.12
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-720
  • 氨基酸序列
    MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA.
  • 基因功能参考文献：
    1. Large domain movements upon UvrD dimerization and helicase activation has been reported. PMID: 29087333
    2. Results show that UvrD monomer translocation rate depends on the single-stranded DNA base composition, even in the absence of any predicted basepairing within the nucleic acid. Translocation is faster on single-stranded DNA-containing pyrimidines and slower on ssDNA-containing purines PMID: 28978435
    3. these data reveal that UvrD exhibits two distinct types of unwinding activity regulated by its stoichiometry. Furthermore, two UvrD conformational states, termed "closed" and "open," correlate with movement toward or away from the DNA fork. PMID: 25883359
    4. The unwinding processivity is increased in the UvrD303 mutant. PMID: 24798324
    5. UvrD binds RNA polymerase during transcription elongation, using its helicase/translocase activity, forces RNA polymerase to slide backward along DNA; UvrD exposes DNA lesions, allowing nucleotide excision repair enzymes to gain access to sites of damage PMID: 24402227
    6. These results suggest that UvrD pauses more frequently during translocation at low ATP but with little futile ATP hydrolysis. PMID: 22342931
    7. The anti-thymineless death role of UvrD. PMID: 21705756
    8. crystal structure of an apo form of UvrD in which the 2B sub-domain is in an "open" state that differs by 160 degrees rotation of the 2B sub-domain PMID: 21704638
    9. Although a 3'-ssDNA partial duplex provides a high-affinity site for a uvrD monomer, the study shows that a monomer also binds with specificity to DNA junctions possessing a 5'-ssDNA flanking region and can initiate translocation from this site. PMID: 20877334
    10. UvrD has a critical role in the processing of DNA-protein cross-links PMID: 20444702
    11. UvrD monomers translocate in discrete steps with an average kinetic step-size, m=3.68 nt step(-1), a translocation rate constant, kt=51.3 steps s(-1), with a processivity corresponding to an average translocation distance of 2400 nt before dissociation PMID: 15561144
    12. mutational analysis of a thermostable UvrD helicase PMID: 15955821
    13. acts by removing RecA or a RecA-made structure, allowing replication fork reversal PMID: 16135232
    14. UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. PMID: 17020578
    15. Role of UvrD on RecET-mediated illegitimate recombination. PMID: 17038801
    16. Our results suggest that macroscopic rate of UvrD monomer translocation is not limited by each ATPase cycle but by a slow step (pause) in each translocation cycle that occurs after four to five rapid 1 nt translocation steps. PMID: 17499041
    17. Results show that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. PMID: 17641684
    18. The multiform patterns of unwinding bursts and the distributions of the off-times favour the mechanism that UvrD unwinds DNA as a dimer. PMID: 19008855

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  • 蛋白家族：
    Helicase family, UvrD subfamily
  • 数据库链接：

    KEGG: ecj:JW3786

    STRING: 316385.ECDH10B_4005