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  4. Recombinant Escherichia coli Ethanolamine utilization protein EutL (eutL)

Recombinant Escherichia coli Ethanolamine utilization protein EutL (eutL)

  • 货号：
    CSB-YP301208ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP301208ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP301208ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP301208ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP301208ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    eutL
  • Uniprot No.：
  • 别名：
    eutL; yffJ; b2439; JW2432Ethanolamine utilization protein EutL
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-219
  • 氨基酸序列
    MPALDLIRPS VTAMRVIASV NADFARELKL PPHIRSLGLI SADSDDVTYI AADEATKQAM VEVVYGRSLY AGAAHGPSPT AGEVLIMLGG PNPAEVRAGL DAMIAHIENG AAFQWANDAQ DTAFLAHVVS RTGSYLSSTA GITLGDPMAY LVAPPLEATY GIDAALKSAD VQLATYVPPP SETNYSAAFL TGSQAACKAA CNAFTDAVLE IARNPIQRA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A component of the bacterial microcompartment (BMC) shell dedicated to ethanolamine degradation (Probable). Two crystal forms have been seen; a form with a closed central pore that has 3 very small (1.1-2.2 Angstroms) channels per trimer lined by acidic and aromatic residues. A form with a large central pore (8-12 Angstroms) has also been seen; this is probably a functional pore which allows molecules to enter and exit the BMC in a selective, gated manner. Another group only sees the central pore in the presence of Zn(2+); soaking crystals in ZnCl(2) leads to dramatic conformational changes that open a central pore of about 12 Angstroms. Whether Zn(2+) binding is physiologically relevant is unclear, however it suggests a gating mechanism exists. Ethanolamine-binding by the small channels has been hypothesized to stabilize the EutL central pore in a closed (non-transporting) state. An open pore is thought to be large enough to transport ATP and/or cobalamin.
  • 基因功能参考文献：
    1. In contrast to EutL, the pore of EutM appears to be positively charged, indicating specificity for different solutes. PMID: 20851901
    2. the crystal structure of the shell protein EutL at 2.2-A resolution is presented. PMID: 19451619
  • 亚细胞定位：
    Bacterial microcompartment.
  • 蛋白家族：
    EutL/PduB family
  • 数据库链接：

    KEGG: ecj:JW2432

    STRING: 316385.ECDH10B_2604