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  4. Recombinant Escherichia coli GTP-dependent nucleic acid-binding protein engD (engD)

Recombinant Escherichia coli GTP-dependent nucleic acid-binding protein engD (engD)

  • 中文名称：
    大肠杆菌ychF重组蛋白
  • 货号：
    CSB-YP364698ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌ychF重组蛋白
  • 货号：
    CSB-EP364698ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌ychF重组蛋白
  • 货号：
    CSB-EP364698ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌ychF重组蛋白
  • 货号：
    CSB-BP364698ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌ychF重组蛋白
  • 货号：
    CSB-MP364698ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ychF
  • Uniprot No.：
  • 别名：
    ychF; engD; gtp1; b1203; JW1194; Ribosome-binding ATPase YchF
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-363
  • 氨基酸序列
    GFKCGIVGL PNVGKSTLFN ALTKAGIEAA NFPFCTIEPN TGVVPMPDPR LDQLAEIVKP QRTLPTTMEF VDIAGLVKGA SKGEGLGNQF LTNIRETEAI GHVVRCFEND NIIHVSGKVN PADDIEVINT ELALADLDTC ERAIHRVQKK AKGGDKDAKA ELAVLEKCLP QLENAGMLRA LDLSAEEKAA IRYLSFLTLK PTMYIANVNE DGFENNPYLD QVREIAAKEG SVVVPVCAAV EADIAELDDE ERDEFMQELG LEEPGLNRVI RAGYKLLNLQ TYFTAGVKEV RAWTIPVGAT APQAAGKIHT DFEKGFIRAQ TISFEDFITY KGEQGAKEAG KMRAEGKDYI VKDGDVMNFL FNV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. Does not hydrolyze GTP.
  • 基因功能参考文献：
    1. Our data provide novel insights into the regulation of the so far ill-defined YchF/Ola1 family of proteins and stipulate their role as negative regulators of the oxidative stress response PMID: 26160547
    2. We have thereby identified the highly conserved His-114 as critical for the ATPase activity of YchF from Escherichia coli. PMID: 26018081
    3. Escherichia coli YchF binds the 50S and 70S ribosomal particles in a nucleotide independent manner and it hydrolyzes ATP utilizing a potassium dependent mechanism. PMID: 21527254
  • 蛋白家族：
    TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family, YchF/OLA1 subfamily
  • 数据库链接：

    KEGG: ecj:JW1194

    STRING: 316385.ECDH10B_1256