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  4. Recombinant Escherichia coli Methyl-accepting chemotaxis protein II (tar), partial

Recombinant Escherichia coli Methyl-accepting chemotaxis protein II (tar), partial

  • 货号：
    CSB-YP356999ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356999ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356999ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356999ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tar
  • Uniprot No.：
  • 别名：
    tar; cheM; b1886; JW1875Methyl-accepting chemotaxis protein II; MCP-II; Aspartate chemoreceptor protein
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for the attractant L-aspartate and related amino and dicarboxylic acids. Tar also mediates taxis to the attractant maltose via an interaction with the periplasmic maltose binding protein. Tar mediates taxis away from the repellents cobalt and nickel.; Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.
  • 基因功能参考文献：
    1. Thus, the proportion of polypeptide chain that is locally and presumably transiently disordered is a structural feature of cytoplasmic domain dynamics that varies with functional region and modification-induced signaling state. PMID: 27318193
    2. There is a differential repositioning of the second transmembrane helices from E. coli Tar and EnvZ upon moving the flanking aromatic residues. PMID: 25445668
    3. the Tar(FO) modules demonstrate that trimerized signaling tips self-associate, bind CheA and CheW, and facilitate conversion of CheA to an active conformation. PMID: 25967982
    4. Residues at the cytoplasmic end of transmembrane helix 2 determine the signal output of the Tar chemoreceptor. PMID: 23495653
    5. Such inversion is enabled by opposing pH sensing by the two major chemoreceptors, Tar and Tsr, such that the relative strength of the response is modulated by adaptive receptor methylation. PMID: 23078189
    6. Here we report the overexpression system for aCB2 in Escherichia coli C43(DE3) facilitated by two fusion partners: Mistic and TarCF, a C-terminal fragment of the bacterial chemosensory transducer Tar at the C-terminal of the CB2 open reading frame region. PMID: 22406258
    7. characterization of structural features of carboxyl-terminal 40 residues of Tar; identifed carboxyl-terminal linker between receptor body and the pentapeptide is an unstructured, disordered segment that can serve as a flexible arm and enzyme tether PMID: 21858888
    8. Ligand specificity is determined by differentially arranged common ligand-binding residues in bacterial amino acid chemoreceptors Tsr and Tar PMID: 21979954
    9. Tar molecules with the cytoplasmic methylation and kinase control domains of Tsr still sensed phenol as an attractant. PMID: 21965561
    10. The cytoplasmic domains of Tar and Tsr receptors are close to each other near the trimer contact region at the cytoplasmic tip. PMID: 21174433
    11. These results with Tar-EnvZ hybrid receptor mutants suggest that intersubunit interactions in the HAMP linker normally mediate signal transduction through both subunits in a sensor dimer. PMID: 15316026
    12. mutations in the protein motifs of Tar significantly reduced the ability of the transmembrane domains to dimerize PMID: 15330757
    13. The tryptophan residues flanking the second transmembrane helix of Tar, especially Trp-209, are crucial in setting the baseline activity and ligand sensitivity of this chemoreceptor. PMID: 15667220
    14. assisted adaptational modification (methylation, demthylation and deamidation) in vitro of Trg chemorecptor PMID: 15916610
    15. polar localization of Tar fused to green fluorescent protein is influenced by its own amidation (methylation) state and the expression of another chemoreceptor (Tsr) PMID: 16267289
    16. Data show that Tar can be readily mutated to respond to new chemical signals, but that the overall change in specificity depends on the target compound. PMID: 16359703
    17. The pentapeptide linker in Tar is important for chemotaxis because of its role in adaptational modification. PMID: 16573695
    18. The fundamental mechanism of transmembrane signaling is conserved between homodimeric sensor kinases (with NarX) and chemoreceptors. PMID: 16707686
    19. Data suggest that Tar()-Tsr* suppression most likely occurs through compensatory changes in the conformation or dynamics of a mixed receptor signaling complex, presumably based on trimer-of-dimer interactions. PMID: 16751275
    20. The organization of these receptor/signaling complexes is determined by conserved interactions between the constituent chemotaxis proteins and may represent the active form in vivo. PMID: 16973743
    21. A mutational analysis of Tar residues 505-548 reveals that the more of this region is deleted, the less sensitive Tar is to inhibition by aspartate. PMID: 19053273

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  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein. Note=Found predominantly at cell poles.
  • 数据库链接：

    KEGG: ecj:JW1875

    STRING: 316385.ECDH10B_2027