Recombinant Escherichia coli Murein hydrolase activator NlpD (nlpD)

This recombinant E.coli K12 nlpD protein is an E.coli-expressed protein (Full Length of Mature Protein). At the theoretical level, the steps needed for obtaining a recombinant protein are pretty straightforward. Obtain the nlpD gene, clone it in an expression vector, transform it into the host of choice, induce it, and then, the protein is ready for purification and characterization. In practice, however, dozens of things can go wrong. Poor growth of the host, inclusion body formation, protein inactivity, and even not obtaining any protein at all are some of the problems often found down the pipeline. So we choose the most suitable strain and vector for this recombinant nlpD protein expression and adopt the most optimized expression condition and purification method. Finally, mild condition and subsequent cumbersome protein-refolding procedures ensure this nlpD protein is soluble.

nlpD is a protein encoding gene that provides an instruction in making a protein named murein hydrolase activator NlpD. The protein encoded by this gene belongs to E.coli NlpD/Haemophilus LppB family. It is similar in sequence to the LppB lipoprotein outer membrane antigen of Haemophilus somnus in Escherichia coli. This protein has metalloendopeptidase activity and is involved in multiple biological processes, including cell cycle, positive regulation of hydrolase activity, response to drug and septum digestion after cytokinesis.