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  4. Recombinant Escherichia coli Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ)

Recombinant Escherichia coli Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ)

  • 中文名称：
    大肠杆菌narJ重组蛋白
  • 货号：
    CSB-YP365240ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌narJ重组蛋白
  • 货号：
    CSB-EP365240ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌narJ重组蛋白
  • 货号：
    CSB-EP365240ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌narJ重组蛋白
  • 货号：
    CSB-BP365240ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌narJ重组蛋白
  • 货号：
    CSB-MP365240ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    narJ
  • Uniprot No.：
  • 别名：
    narJ; b1226; JW1217; Nitrate reductase molybdenum cofactor assembly chaperone NarJ; Redox enzyme maturation protein NarJ
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-236
  • 氨基酸序列
    MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA QYSELFDRGR ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY LAQLPQSEAV EGLKDIAPIL ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD EARDDTPQAL DAVWEEEQVK FFADKGCGDS AITAHQRRFA GAVAPQYLNI TTGGQH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane.
  • 基因功能参考文献：
    1. the NarJ chaperone interacts at two distinct binding sites of the apoenzyme PMID: 16286471
    2. The interactome of NarJ exhibits Tat-dependence. PMID: 16540088
    3. Data demonstrate that NarJ has a multifunctional role in nitrate reductase A biosynthesis, as it orchestrates both the maturation and the assembly steps. PMID: 17442677
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    NarJ/NarW family
  • 数据库链接：

    KEGG: ecj:JW1217

    STRING: 316385.ECDH10B_1285