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  4. Recombinant Escherichia coli Nitrogen regulatory protein (ptsN)

Recombinant Escherichia coli Nitrogen regulatory protein (ptsN)

  • 货号：
    CSB-YP301715ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP301715ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP301715ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP301715ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP301715ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ptsN
  • Uniprot No.：
  • 别名：
    ptsN; rpoP; yhbI; b3204; JW3171; Nitrogen regulatory protein; Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-163
  • 氨基酸序列
    MTNNDTTLQL SSVLNRECTR SRVHCQSKKR ALEIISELAA KQLSLPPQVV FEAILTREKM GSTGIGNGIA IPHGKLEEDT LRAVGVFVQL ETPIAFDAID NQPVDLLFAL LVPADQTKTH LHTLSLVAKR LADKTICRRL RAAQSDEELY QIITDTEGTP DEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Seems to have a role in regulating nitrogen assimilation.
  • 基因功能参考文献：
    1. Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation. PMID: 23517463
    2. Here the s show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG. PMID: 21531803
    3. PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level. PMID: 21143318
    4. The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy. PMID: 15741344
    5. the dephospho-form of enzyme IIA(Ntr) is required for derepression of the ilvBN operon encoding acetohydroxy acid synthase I catalysing the first step common to the biosynthesis of the branched-chain amino acids. PMID: 16164569
    6. overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E). PMID: 18253509
    7. EIIANtr is readily phosphorylated at the histidine 73. PMID: 18625021

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ecj:JW3171

    STRING: 316385.ECDH10B_3378