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  4. Recombinant Escherichia coli Nitrogen regulatory protein P-II 2 (glnK)

Recombinant Escherichia coli Nitrogen regulatory protein P-II 2 (glnK)

  • 中文名称：
    大肠杆菌glnK重组蛋白
  • 货号：
    CSB-YP359722ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌glnK重组蛋白
  • 货号：
    CSB-EP359722ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌glnK重组蛋白
  • 货号：
    CSB-EP359722ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌glnK重组蛋白
  • 货号：
    CSB-BP359722ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌glnK重组蛋白
  • 货号：
    CSB-MP359722ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    glnK
  • Uniprot No.：
  • 别名：
    glnK; ybaI; b0450; JW0440Nitrogen regulatory protein P-II 2
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-112
  • 氨基酸序列
    MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI RIRTGEADEA AL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the regulation of nitrogen metabolism. Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell. Involved in the regulation of the ammonium transporter AmtB so as to optimize ammonium uptake under all growth conditions. In nitrogen-limited conditions, GlnK does not interact with AmtB, which remains active and imports ammonium. When extracellular ammonium increases, GlnK associates tightly with AmtB in the inner membrane, thereby inhibiting the transporter activity. Also involved in the regulation of the glutamine synthetase adenylyltransferase/adenylyl-removing (AT/AR) enzyme GlnE and the glutamine synthetase GlnA. In nitrogen-limited conditions, formation of uridylylated GlnB(PII)/GlnK heterotrimers may fine-regulate the activation of GlnA: uridylylated heterotrimers stimulate the deadenylation and the activation of GlnA whereas uridylylated GlnK homotrimers do not stimulate, or hardly stimulate, the deadenylation of GlnA. In addition, regulates the expression of Ntr genes during nitrogen starvation, probably via the control of the levels of phosphorylated NRI during different stages of the response to nitrogen limitation.
  • 基因功能参考文献：
    1. Results show that GlnK is tightly regulated under nitrogen-rich conditions, yet it is expressed during ammonium run-out and starvation. This suggests a role for GlnK as a buffer of nitrogen shock after starvation, and provides a further functional link between nitrogen and carbon metabolisms. PMID: 28538158
    2. Data show that the P(II) protein, GlnK, has an ATPase activity that is inhibited by 2-oxoglutarate. PMID: 23818625
    3. Studies suggest a hypothesise that the regulatory protein GlnK is required to fine-tune the active transport of ammonium in order to limit futile cycling whilst enabling an intracellular ammonium level sufficient for the cell's nitrogen requirements. PMID: 21134373
    4. Co-purification of GlnK with AmtB from ammonium-shocked E. coli cells definitvely demonstrates that these two proteins form a functional complex with a stoichiometry of 1:1 and dependent on the uridylylation status of GlnK. PMID: 16864585
    5. Data show a subtle interplay between GlnB and GlnK. PMID: 19438718

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  • 亚细胞定位：
    Cytoplasm. Cell inner membrane.
  • 蛋白家族：
    P(II) protein family
  • 数据库链接：

    KEGG: ecj:JW0440

    STRING: 316385.ECDH10B_0406