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  4. Recombinant Escherichia coli Nuclease sbcCD subunit D (sbcD)

Recombinant Escherichia coli Nuclease sbcCD subunit D (sbcD)

  • 货号：
    CSB-YP313428ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313428ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP313428ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313428ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313428ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sbcD
  • Uniprot No.：
  • 别名：
    sbcD; yajA; b0398; JW0388; Nuclease SbcCD subunit D
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-400
  • 氨基酸序列
    MRILHTSDWH LGQNFYSKSR EAEHQAFLDW LLETAQTHQV DAIIVAGDVF DTGSPPSYAR TLYNRFVVNL QQTGCHLVVL AGNHDSVATL NESRDIMAFL NTTVVASAGH APQILPRRDG TPGAVLCPIP FLRPRDIITS QAGLNGIEKQ QHLLAAITDY YQQHYADACK LRGDQPLPII ATGHLTTVGA SKSDAVRDIY IGTLDAFPAQ NFPPADYIAL GHIHRAQIIG GMEHVRYCGS PIPLSFDECG KSKYVHLVTF SNGKLESVEN LNVPVTQPMA VLKGDLASIT AQLEQWRDVS QEPPVWLDIE ITTDEYLHDI QRKIQALTES LPVEVLLVRR SREQRERVLA SQQRETLSEL SVEEVFNRRL ALEELDESQQ QRLQHLFTTT LHTLAGEHEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.
  • 基因功能参考文献：
    1. Data show that replication forks form a palindrome-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and exonuclease I (ExoI) before chromosome replication can be completed. PMID: 29208713
    2. The results suggest that SbcCD processes hairpin and linear dsDNA ends with a novel DNA end-dependent binary endonuclease activity in response to substrate length. PMID: 26319016
    3. The SbcD monomer exhibits single-stranded DNA endonuclease activity and double-stranded DNA exonuclease activity. PMID: 24531464
    4. In sbcCD mutants, RecA4142 can bind other DNA substrates by itself that are normally degraded by the SbcCD nuclease. PMID: 20304994
    5. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein, and SbcD protein is distributed through the cytoplasm. PMID: 17644583
    6. The absence of SbcC and SbcD in sbcB15 cells decreased stationary-phase mutation. PMID: 19018518
    7. Study found that functions of SbcCD, a DNA structure specific nuclease, were needed in the RNase E-dependent lacZ expression of the GAA repeats. PMID: 19733517

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  • 蛋白家族：
    SbcD family
  • 数据库链接：

    KEGG: ecj:JW0388

    STRING: 316385.ECDH10B_0354