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  4. Recombinant Escherichia coli Osmolarity sensor protein EnvZ (envZ), partial

Recombinant Escherichia coli Osmolarity sensor protein EnvZ (envZ), partial

  • 中文名称：
    大肠杆菌envZ重组蛋白
  • 货号：
    CSB-YP365158ENV1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌envZ重组蛋白
  • 货号：
    CSB-EP365158ENV1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌envZ重组蛋白
  • 货号：
    CSB-EP365158ENV1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌envZ重组蛋白
  • 货号：
    CSB-BP365158ENV1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌envZ重组蛋白
  • 货号：
    CSB-MP365158ENV1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    envZ
  • Uniprot No.：
  • 别名：
    envZ; ompB; perA; tpo; b3404; JW3367; Sensor histidine kinase EnvZ; Osmolarity sensor protein EnvZ
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system EnvZ/OmpR involved in osmoregulation (particularly of genes ompF and ompC) as well as other genes. EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals; at low osmolarity OmpR activates ompF transcription, while at high osmolarity it represses ompF and activates ompC transcription. Also dephosphorylates OmpR in the presence of ATP. The cytoplasmic dimerization domain (CDD) forms an osmosensitive core; increasing osmolarity stabilizes this segment (possibly by its contraction), enhancing the autophosphorylation rate and consequently, downstream phosphotransfer to OmpR and signaling. Autophosphorylation is greater when full-length EnvZ is reconstituted in a lipid environment, lipid-mediated allostery impacts the kinase function of EnvZ. Involved in acid stress response; this requires EnvZ but not OmpR phosphorylation, and suggests that EnvZ senses cytoplasmic acidic pH.
  • 基因功能参考文献：
    1. study also reveals that polyP-dependent stalling has little effect on EnvZ levels. Hence, both polyP motifs in EnvZ are primarily involved in protein-protein interaction. PMID: 29953503
    2. A library of single-Cys-containing EnvZ proteins was used to show that the major TM1-TM1' interface falls along a single surface consisting of residue positions 19, 23, 26, 30 and 34. Cys substitutions in the N- and C-terminal regions of TM1 change EnvZ signal output. Core residues in TM1 cause TM1 dimerisation and maintenance of steady-state signal output. Extracellular osmolarity does not rearrange TM1-TM1' interfaces. PMID: 27155567
    3. Cytoplasmic sensing by the inner membrane histidine kinase EnvZ PMID: 25937465
    4. structure shows an asymmetric histidine kinase dimer where one subunit is caught performing the autophosphorylation reaction PMID: 24500224
    5. Both the E. coli MG1655 and E. coli Nissle 1917 strains containing envZP41L produced more phosphorylated OmpR than their parents. PMID: 24478082
    6. HDXMS data showed that the cytoplasmic domain of EnvZ exists as an ensemble of multiple conformations and osmolytes favoured increased helicity. High osmolality increased the autophosphorylation of His243. PMID: 22543870
    7. This study report that 10 to 20% of motile E. coli MG1655 are envZ missense mutants that are better colonizers of the mouse intestine than E. coli MG1655. PMID: 22392928
    8. Direct binding studies showed that the cytoplasmic domains of these proteins each form specific homodimers in vitro. PMID: 21854787
    9. Together, the data suggest that the binding of MzrA to EnvZ influences the ability of EnvZ to receive and/or respond to environmental signals in the periplasm and modulate its biochemical output to OmpR. PMID: 20889743
    10. Data show that PerA is required for activation of the operon LEE1, but that this activation is via autoactivation of the per promoter which ensures sufficient production of the PerC protein to activate LEE1. PMID: 15522091

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  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: ecj:JW3367

    STRING: 316385.ECDH10B_3579