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  4. Recombinant Escherichia coli Peptidyl-tRNA hydrolase (pth)

Recombinant Escherichia coli Peptidyl-tRNA hydrolase (pth)

  • 中文名称：
    大肠杆菌pth重组蛋白
  • 货号：
    CSB-EP358958ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    pth; b1204; JW1195; Peptidyl-tRNA hydrolase; PTH; EC 3.1.1.29
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    25.1kDa
  • 表达区域：
    1-194aa
  • 氨基酸序列
    MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAKFFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQKLIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death.
  • 基因功能参考文献：
    1. Study identifies the amino acid residues involved in the tRNA moiety recognition and also revealed the mechanism by which Pth accepts the diverse sequences of the elongator-tRNAs as substrate components. PMID: 22923517
    2. crystals of peptidyl-tRNA hydrolase belonged to the hexagonal space group P6(1), with unit-cell parameters a = b = 55.1, c = 413.1 A PMID: 22139168
    3. Possible mechanism for the suppression of thermosensitivity of pth(Ts) mutants through tRNALys overproduction. PMID: 16540595
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    PTH family
  • 数据库链接：

    KEGG: ecj:JW1195

    STRING: 316385.ECDH10B_1257