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  4. Recombinant Escherichia coli Probable rRNA maturation factor YbeY (ybeY)

Recombinant Escherichia coli Probable rRNA maturation factor YbeY (ybeY)

  • 中文名称：
    大肠杆菌ybeY重组蛋白
  • 货号：
    CSB-YP359098ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌ybeY重组蛋白
  • 货号：
    CSB-EP359098ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌ybeY重组蛋白
  • 货号：
    CSB-EP359098ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌ybeY重组蛋白
  • 货号：
    CSB-BP359098ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌ybeY重组蛋白
  • 货号：
    CSB-MP359098ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ybeY
  • Uniprot No.：
  • 别名：
    ybeY; b0659; JW0656Endoribonuclease YbeY; EC 3.1.-.-
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-155
  • 氨基酸序列
    MSQVILDLQL ACEDNSGLPE ESQFQTWLNA VIPQFQEESE VTIRVVDTAE SHSLNLTYRG KDKPTNVLSF PFEVPPGMEM SLLGDLVICR QVVEKEAQEQ GKPLEAHWAH MVVHGSLHLL GYDHIEDDEA EEMEALETEI MLALGYEDPY IAEKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'- and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.
  • 基因功能参考文献：
    1. This work provides the first evidence that YbeY is recruited to the ribosome through interaction with proteins involved in ribosome biogenesis (i.e., ribosomal protein S11, Era). PMID: 27834201
    2. This observation suggests that the function of HuYbeY in human cells is likely regulated through specific interactions with partner proteins similarly to the way YbeY is regulated in bacteria. PMID: 28153719
    3. evidence which tie YbeY to the transcription antitermination process PMID: 23638028
    4. Deletion of ybeY strongly affects the maturation of rRNA, and causes striking defects in ribosome function including decreased ribosome activity, reduced translational fidelity and altered translation initiation factor binding. PMID: 20807199
    5. Data show that the in vitro activity of the translation machinery of ybeY deletion mutants is significantly lower than that of the wild type. PMID: 20639334
    6. ybeY protein from Escherichia coli is a metalloprotein PMID: 16511207
    7. The s provide evidence that YbeY, a conserved heat shock protein with unknown function, is involved in the translation process. PMID: 19181801

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Endoribonuclease YbeY family
  • 数据库链接：

    KEGG: ecj:JW0656

    STRING: 316385.ECDH10B_0728