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  4. Recombinant Escherichia coli Proline/betaine transporter (proP), partial

Recombinant Escherichia coli Proline/betaine transporter (proP), partial

  • 货号：
    CSB-YP314757ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP314757ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP314757ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP314757ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP314757ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    proP
  • Uniprot No.：
  • 别名：
    proP; b4111; JW4072; Proline/betaine transporter; Proline porter II; PPII
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in the bacterial osmoregulatory response.
  • 基因功能参考文献：
    1. molecular model predicts the presence of a proton pathway within the N-terminal six-helix bundle of ProP PMID: 15823022
    2. ProP osmotic activation is tuned by both its C-terminal coiled-coil and osmotically induced changes in phospholipid composition PMID: 16239220
    3. These studies define position of ProP TM XII within the membrane, further support predicted structure of ProP, reveal the dimerization interface, and show that the structure of transmembrane segment XII influences the osmolality at which ProP activates. PMID: 17441691
    4. Association of proP with cardiolipin (and not phosphatidylglycerol) may be required for polar ProP localization. PMID: 17504273
    5. ProP switches among "off", "on", and "osmolality-sensitive" states as the membrane potential increases. PMID: 18620422
    6. Periplasmic loops of osmosensory transporter ProP in Escherichia coli are sensitive to osmolality PMID: 19049385
    7. ProP adopts a highly alpha-helical fold similar to that of LacY, and both transmembrane folds exhibit unusually high temperature-sensitive solvent accessibility PMID: 19366597

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  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family
  • 数据库链接：

    KEGG: ecj:JW4072

    STRING: 316385.ECDH10B_4303