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  4. Recombinant Escherichia coli RNA polymerase sigma-E factor (rpoE)

Recombinant Escherichia coli RNA polymerase sigma-E factor (rpoE)

  • 货号：
    CSB-YP314854ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP314854ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP314854ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP314854ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP314854ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoE
  • Uniprot No.：
  • 别名：
    rpoE; sigE; b2573; JW2557; ECF RNA polymerase sigma-E factor; RNA polymerase sigma-E factor; Sigma-24
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-191
  • 氨基酸序列
    MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFRGDSAF YTWLYRIAVN TAKNYLVAQG RRPPSSDVDA IEAENFESGG ALKEISNPEN LMLSEELRQI VFRTIESLPE DLRMAITLRE LDGLSYEEIA AIMDCPVGTV RSRIFRAREA IDNKVQPLIR R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane. Indirectly activates transcription of csrB and csrC, 2 sRNAs that antagonize translational regulator CsrA, linking envelope stress, the stringent response and the catabolite repression systems.
  • 基因功能参考文献：
    1. Mapping of 5' ends of rpoE mRNA identified five new transcriptional initiation sites (P1 to P5) located distal to Esigma(E)-regulated promoter. PMID: 27629414
    2. These results demonstrate that appropriate regulation of sigma(E) activity, ensuring that it is neither too high nor too low, is critical for envelope integrity and cell viability. PMID: 28373273
    3. This paper describes a unique mutation that enhances kinetics of the rpoE envelope stress response pathway rather than preinducing the response. PMID: 27325680
    4. In particular, Esigma(S) appears to contribute significantly to transcription of genes encoding proteins involved in LPS biosynthesis and in cell surface composition. Finally, our results highlight a direct role of Esigma(S) in the regulation of non coding RNAs PMID: 26020590
    5. RpoE regulates transcription of the defective prophage DLP12 lysis genes through interaction with QDLP12. PMID: 25998262
    6. However, membrane protein damage induced by gentamicin can be eliminated or repaired by RpoE- and Cpx-dependent mechanisms pre-emptively induced in P-starved cells. PMID: 25039943
    7. TraR increases the concentrations of periplasmic chaperones and proteases by directly activating the transcription of sigma(E)-dependent promoters. PMID: 25535270
    8. The sigma(E) response was activated in Deltahfq mutants of all strains tested, resulting from the misregulation ofouter membrane proteins, whereas the Cpx response was activated by loss of hfq in enteropathogenic strains, but not in E. coli K-12. PMID: 24628810
    9. Through employing a labile mutant periplasmic protein, AcrA(L222Q), it was determined that the rpoE3 mutation overcomes the DeltadegP phenotypes, in part, by activating a sigma(E)-dependent proteolytic pathway. PMID: 22439016
    10. Taken together, the results suggest that the cell lysis proceeds in the cascade of sigma(E) --> expression of micA and rybB --> reduction in Omp proteins --> disintegration of the outer membrane. PMID: 22251246
    11. Recognition of beta-strand motifs of sigma(E) by RseB is required for sigma(E) activity. PMID: 21908666
    12. RpoE is required for stress-induced lac amplification. The role of the sigma(E) stress response in mutagenesis is independent of SOS and DinB upregulation, cytoplasmic heat-shock- and RpoS-stress responses. PMID: 20497332
    13. Role in resistance to zinc, cadmium, and copper toxicity. PMID: 15774872
    14. hypotheses:(1)negatively charged N-terminal domain of sigma(70) is bound in polymerase;(2)rate-determining isomerization involves ejecting this domain, unmasking active site; (3)conversion to RP(o) involves coupled folding of downstream clamp of pol. PMID: 16475805
    15. Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli. PMID: 17158661
    16. the PDZ domain and loop L3 of DegS define a novel molecular switch allowing strict regulation of the sigmaE stress response system PMID: 17938245
    17. when sigma(E) is inhibited, cell envelope stress increases and envelope integrity is lost PMID: 18253509

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, ECF subfamily
  • 数据库链接：

    KEGG: ecj:JW2557

    STRING: 316385.ECDH10B_2741