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  4. Recombinant Escherichia coli Ribonuclease HI (rnhA)

Recombinant Escherichia coli Ribonuclease HI (rnhA)

  • 货号：
    CSB-YP359051ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP359051ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP359051ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP359051ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP359051ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rnhA
  • Uniprot No.：
  • 别名：
    rnhA; dasF; herA; rnh; sdrA; b0214; JW0204; Ribonuclease HI; RNase HI; EC 3.1.26.4; Ribonuclease H; RNase H
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-155
  • 氨基酸序列
    MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERCDELARAA AMNPTLEDTG YQVEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.
  • 基因功能参考文献：
    1. Data show that the catalytic domains of E. coli and human RNase H have nearly identical sequence preferences, which correlate with the efficiency of RNase H-recruiting antisense oligonucleotides. PMID: 29126318
    2. RNase HI recruits RnlB to RnlA through N-repeated domain for inhibiting RnlA toxicity and thus plays two contrary roles in the regulation of RnlA-RnlB system. PMID: 28085056
    3. Here, s provide the evidence that RNase HI is an essential factor for activation of RnlA. PMID: 24308852
    4. overproduction compensates for absence of topoisomerase I in RNA synthesis PMID: 15458416
    5. crystal structure of ribonuclease HI PMID: 15644213
    6. report of the complete force-induced unfolding and refolding trajectory of a single molecule of RNase H with the use of force-measuring optical tweezers PMID: 16179479
    7. Results describe the unfolded state ensemble of RNAse H. PMID: 16426636
    8. Adjusting conformational changes within RNase H for activity at high temperatures. PMID: 17088323
    9. RNase HI plays a more central role in DNA topology than previously thought. PMID: 18554330
    10. Data show that overproduction of RNase HI limits the accumulation of hypernegative supercoiling, thereby preventing extensive RNA degradation. PMID: 18790862
    11. a single change at Ile25 (I25A) resulted in the equilibrium population of the RNase H intermediate under near-native conditions. PMID: 19505477

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    RNase H family
  • 数据库链接：

    KEGG: ecj:JW0204

    STRING: 316385.ECDH10B_0195