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  4. Recombinant Escherichia coli Ribose-5-phosphate isomerase A (rpiA)

Recombinant Escherichia coli Ribose-5-phosphate isomerase A (rpiA)

  • 中文名称：
    大肠杆菌rpiA重组蛋白
  • 货号：
    CSB-YP541334ENX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌rpiA重组蛋白
  • 货号：
    CSB-EP541334ENX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌rpiA重组蛋白
  • 货号：
    CSB-EP541334ENX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌rpiA重组蛋白
  • 货号：
    CSB-BP541334ENX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌rpiA重组蛋白
  • 货号：
    CSB-MP541334ENX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpiA
  • Uniprot No.：
  • 别名：
    rpiA; EcSMS35_3047; Ribose-5-phosphate isomerase A; EC 5.3.1.6; Phosphoriboisomerase A; PRI
  • 种属：
    Escherichia coli (strain SMS-3-5 / SECEC)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-219
  • 氨基酸序列
    MTQDELKKAV GWAALQYVQP GTIVGVGTGS TAAHFIDALG TMKGQIEGAV SSSDASTEKL KSLGIHVFDL NEVDSLGIYV DGADEINGHM QMIKGGGAAL TREKIIASVA EKFICIADAS KQVDILGKFP LPVEVIPMAR SAVARQLVKL GGRPEYRQGV VTDNGNVILD VHGMEILDPI AMENAINAIP GVVTVGLFAN RGADVALIGT PDGVKTIVK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am looking to buy the protein RPI-A with an N-terminal HIS-tag. As I work with highly sensitive aptamer tools, I need to know the exact sequence before I buy. Is it only the protein plus 6xHIS, or is there a linker contained as well? If so, do you know if it impacts the structure?

A:
Thanks for your inquiry.
Recombinant Escherichia coli Ribose-5-phosphate isomerase A(rpiA)
Express Region: 1-219aa; Full length protein.
Tag information:We are not sure you need C terminal 6*his tag or N terminal 6*his tag. We will try to link to the tag you want in prior, if we can't provide it, we could provide other tags. ( The success rate is 95.25%）
Sequence:

MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKGQIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIVK


If you need 6*his, there will be no linker. If you need the fusion protein with other tags, there will be linkers. Usually it doesn't impact the structurer.
Shipping format: The default delivery form is liquid form. If you have demand for lyophilized form, please remark this requirement when placing order.
Optional service: Do you want aseptic processing and/or endotoxin removal for this protein (recommended for cell culture or in vivo use)? With our endotoxin removal service we guarantee the endotoxin level to be less than 0.1ng/ug (1EU ug). Both aseptic processing and endotoxin removal services are free of charge however they must be requested when the order is placed.
Western Blot service: We could offer Western Blot service for tag antibody free of charge. If you have demand, please remark this information when placing the order.
Shipping condition: Our proteins are routinely shipped on ice packs.

靶点详情

  • 功能：
    Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.
  • 蛋白家族：
    Ribose 5-phosphate isomerase family
  • 数据库链接：

    KEGG: ecm:EcSMS35_3047