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  4. Recombinant Escherichia coli Ribosome modulation factor (rmf)

Recombinant Escherichia coli Ribosome modulation factor (rmf)

  • 中文名称：
    大肠杆菌rmf重组蛋白
  • 货号：
    CSB-YP365366ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌rmf重组蛋白
  • 货号：
    CSB-EP365366ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌rmf重组蛋白
  • 货号：
    CSB-EP365366ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌rmf重组蛋白
  • 货号：
    CSB-BP365366ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌rmf重组蛋白
  • 货号：
    CSB-MP365366ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rmf
  • Uniprot No.：
  • 别名：
    rmf; b0953; JW0936; Ribosome modulation factor; RMF; Hibernation factor RMF; Protein E
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-55
  • 氨基酸序列
    MKRQKRDRLE RAHQRGYQAG IAGRSKEMCP YQTLNQRSQW LGGWREAMAD RVVMA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    During stationary phase, converts 70S ribosomes to an immature dimeric form (90S ribosomes) which are converted to inactive 100S ribosomes (a process called ribosomal hibernation) by the hibernation promoting factor HPF. Inactivates ribosomes by covering the peptidyl transferase (PTase) center of the 23S rRNA and the entrance of peptide exit tunnel. However crystallization with T.thermophilus 70S ribosomes shows it binds near the 3'-end of the 16S rRNA near the anti-Shine-Dalgarno sequence, where it would sterically hinder translation inititation. In this crystal binding of RMF induces movement of the 30S head domain away from the rest of the ribosome, presumably so they would more easily form dimers. Also involved in protection against heat stress, but this role is not dependent on the maintenance of ribosome dimers.
  • 基因功能参考文献：
    1. Data propose that CRP regulates transcription activation of the rmf gene for formation of 100S ribosome dimers. PMID: 23475967
    2. crystal structure of Thermus thermophilus 70S ribosome in complex with stationary-phase factors RMF, HPF and YfiA; binding site of RMF overlaps with that of mRNA Shine-Dalgarno sequence, which prevents interaction between the mRNA and the 16S ribosomal RNA PMID: 22605777
    3. Ribosomes change into forms which are hard to bind with IF3 and easy to form 100S ribosomes by RMF and HPF concomitantly with transition from the log phase to the stationary phase. PMID: 19170772
    4. Data showed that FtsY depletion inhibits the translation of both SRP-dependent and independent proteins. This synthesis defect is the result of a multifaceted response that includes the upregulation of the ribosome-inactivating protein RMF. PMID: 19749044
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Ribosome modulation factor family
  • 数据库链接：

    KEGG: ecj:JW0936

    STRING: 316385.ECDH10B_1023