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  4. Recombinant Escherichia coli Ribosome-recycling factor (frr)

Recombinant Escherichia coli Ribosome-recycling factor (frr)

  • 货号：
    CSB-YP014941ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014941ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014941ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014941ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014941ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    frr
  • Uniprot No.：
  • 别名：
    frr; rrf; b0172; JW0167; Ribosome-recycling factor; RRF; Ribosome-releasing factor
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-185
  • 氨基酸序列
    MISDIRKDAE VRMDKCVEAF KTQISKIRTG RASPSLLDGI VVEYYGTPTP LRQLASVTVE DSRTLKINVF DRSMSPAVEK AIMASDLGLN PNSAGSDIRV PLPPLTEERR KDLTKIVRGE AEQARVAVRN VRRDANDKVK ALLKDKEISE DDDRRSQDDV QKLTDAAIKK IEAALADKEA ELMQF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.
  • 基因功能参考文献：
    1. Ribosome recycling after peptide chain termination is a special case of the general RRF and EF-G function, which happens whenever fMet-tRNA is not available for triggering 70S scanning. PMID: 27261258
    2. Data show that monosomes produced in ribosome recycling factor-deficient cells are able to translate synthetic homopolymer but not natural mRNA with leader and canonical initiation signal. PMID: 15522083
    3. data enable a model to be proposed for the dual action of EF-G and RRF during ribosome recycling PMID: 15616575
    4. domain II of RRF plays a crucial role in the concerted action of RRF and EF-G for the post-termination complex disassembly, and the specific interaction between RRF and EF-G on ribosomes mainly depends on the interaction between domain II of RRF and EF-G PMID: 16262604
    5. V117D mutation does not perturb the efficient interdomain interaction, which results in cooperative unfolding of the RRF protein. PMID: 16684502
    6. RRF is capable of spontaneously moving from its initial binding site on the 70S ribosome to a site exclusively on the large 50S ribosomal subunit. PMID: 17643374
    7. The disruption of bridge B2a upon RRF binding to the ribosome seen in the present structures reveals one of the key roles that RRF plays in ribosome recycling, the dissociation of 70S ribosomes into subunits. PMID: 18234219

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    RRF family
  • 数据库链接：

    KEGG: ecj:JW0167

    STRING: 316385.ECDH10B_0152