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  4. Recombinant Escherichia coli Sec-independent protein translocase protein TatA (tatA), partial

Recombinant Escherichia coli Sec-independent protein translocase protein TatA (tatA), partial

  • 货号：
    CSB-YP300837ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP300837ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP300837ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP300837ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP300837ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tatA
  • Uniprot No.：
  • 别名：
    tatA; mttA1; yigT; b3836; JW3813; Sec-independent protein translocase protein TatA
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.
  • 基因功能参考文献：
    1. The s observe that substitutions of charged residues located in the TatA amphipathic helix lock TatA in an assembled state, suggesting that these charged residues play a critical role in the protein translocation step that follows TatA assembly. PMID: 28857741
    2. Live cell imaging shows reversible assembly of the TatA component of the twin-arginine protein transport system. PMID: 24003141
    3. Each subunit of TatA consists of a transmembrane segment, an amphiphilic helix (APH), and a C-terminal densely charged region (DCR). The sequence of charges in the DCR is complementary to the charge pattern on the APH, suggesting that the protein can be "zipped up" by a ladder of seven salt bridges. PMID: 23332763
    4. analysis of the Tat translocation mechanism through structural and biophysical studies of the amphipathic region of TatA from Escherichia coli PMID: 21683683
    5. the TatA protein represents a bifunctional component fulfilling both the TatA and TatB functions. PMID: 15557327
    6. TatA fused to green/cyan/yellow fluorescent protein is found to be preferentially incorporated into the TatABC complex. PMID: 15728576
    7. TatA may be the major pore-forming subunit in the twin-arginine translocation pathway [review] PMID: 15748976
    8. We used purified preproteins to study interaction with the phospholipid membrane by using surface plasmon resonance.Preproteins interact tightly with a model membrane consisting of only phospholipids in early step in protein translocation by Tat system. PMID: 16475812
    9. it is only in TatA that the amphipathic helix is sensitive to amino acid substitutions; the TatA amphipathic helix may play a role in forming and controlling the path of substrate movement across the membrane PMID: 17565984
    10. data support the TatA polymerization model for the mechanism of Tat transport. PMID: 18832162

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  • 亚细胞定位：
    Cell inner membrane; Single-pass membrane protein; Cytoplasmic side. Note=Abundant all over the membrane, but is more concentrated at the cell poles.
  • 蛋白家族：
    TatA/E family
  • 数据库链接：

    KEGG: ecj:JW3813

    STRING: 316385.ECDH10B_4027