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  4. Recombinant Escherichia coli Selenocysteine-specific elongation factor (selB)

Recombinant Escherichia coli Selenocysteine-specific elongation factor (selB)

  • 货号：
    CSB-YP007436ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007436ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007436ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007436ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007436ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    selB
  • Uniprot No.：
  • 别名：
    selB; fdhA; b3590; JW3563; Selenocysteine-specific elongation factor; SelB translation factor
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-614
  • 氨基酸序列
    MIIATAGHVD HGKTTLLQAI TGVNADRLPE EKKRGMTIDL GYAYWPQPDG RVPGFIDVPG HEKFLSNMLA GVGGIDHALL VVACDDGVMA QTREHLAILQ LTGNPMLTVA LTKADRVDEA RVDEVERQVK EVLREYGFAE AKLFITAATE GRGMDALREH LLQLPEREHA SQHSFRLAID RAFTVKGAGL VVTGTALSGE VKVGDSLWLT GVNKPMRVRA LHAQNQPTET ANAGQRIALN IAGDAEKEQI NRGDWLLADV PPEPFTRVIV ELQTHTPLTQ WQPLHIHHAA SHVTGRVSLL EDNLAELVFD TPLWLADNDR LVLRDISARN TLAGARVVML NPPRRGKRKP EYLQWLASLA RAQSDADALS VHLERGAVNL ADFAWARQLN GEGMRELLQQ PGYIQAGYSL LNAPVAARWQ RKILDTLATY HEQHRDEPGP GRERLRRMAL PMEDEALVLL LIEKMRESGD IHSHHGWLHL PDHKAGFSEE QQAIWQKAEP LFGDEPWWVR DLAKETGTDE QAMRLTLRQA AQQGIITAIV KDRYYRNDRI VEFANMIRDL DQECGSTCAA DFRDRLGVGR KLAIQILEYF DRIGFTRRRG NDHLLRDALL FPEK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
  • 基因功能参考文献：
    1. SelB is involved in translational regulation of cytotoxic necrotizing factor 1(CNF1) expression but without incorporation of selenocysteine in CNF1 protein. PMID: 21570972
    2. analysis of the thermodynamic and kinetic framework of selenocysteyl-tRNASec recognition by elongation factor SelB PMID: 19940162
    3. structures show that tandem winged-helix motifs not only provide an excellent scaffold for RNA binding but can also have an active role in the function of protein-RNA complexes PMID: 17537456
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, SelB subfamily
  • 数据库链接：

    KEGG: ecj:JW3563

    STRING: 316385.ECDH10B_3771