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  4. Recombinant Escherichia coli Spheroplast protein Y (spy)

Recombinant Escherichia coli Spheroplast protein Y (spy)

  • 货号：
    CSB-YP300608ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP300608ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP300608ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP300608ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP300608ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    spy
  • Uniprot No.：
  • 别名：
    spy; b1743; JW1732; Periplasmic chaperone Spy; Spheroplast protein Y
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-161
  • 氨基酸序列
    ADTTTAA PADAKPMMHH KGKFGPHQDM MFKDLNLTDA QKQQIREIMK GQRDQMKRPP LEERRAMHDI IASDTFDKVK AEAQIAKMEE QRKANMLAHM ETQNKIYNIL TPEQKKQFNA NFEKRLTERP AAKGKMPATA E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface. Substrate protein folds while it is bound to chaperone. Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity. Overexpression enhances the stability of otherwise unstable periplasmic proteins.
  • 基因功能参考文献：
    1. Visualizing of Spy chaperone-assisted Im7 protein folding has been reported. PMID: 27239796
    2. Experimental results on Spy-Im7 interactions were used to construct a model for how this chaperone interacts with its clients. It appears that Spy binds to and releases client proteins in a regulated fashion that uses client protein folding as its own cue for release. PMID: 27293188
    3. Spy rapidly associates with its substrate, immunity protein 7 (Im7), thereby eliminating Im7's potential for aggregation. PMID: 26619265
    4. Establish a good relationship between the degree of instability and the improvement chaperone activity of spy variants. PMID: 24497545
    5. Report of the crystal structure of Escherichia coli spheroplast protein y (EcSpy). PMID: 20799348
    6. The induction of spy gene transcription by external zinc may include multiple steps such as through sensing the zinc-induced envelope disorder by BaeSR. PMID: 17884222

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  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    CpxP/Spy family
  • 数据库链接：

    KEGG: ecj:JW1732

    STRING: 316385.ECDH10B_1881