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  4. Recombinant Escherichia coli Transcription elongation protein nusA (nusA)

Recombinant Escherichia coli Transcription elongation protein nusA (nusA)

  • 中文名称：
    大肠杆菌nusA重组蛋白
  • 货号：
    CSB-YP365291ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌nusA重组蛋白
  • 货号：
    CSB-EP365291ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌nusA重组蛋白
  • 货号：
    CSB-EP365291ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌nusA重组蛋白
  • 货号：
    CSB-BP365291ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌nusA重组蛋白
  • 货号：
    CSB-MP365291ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    nusA
  • Uniprot No.：
  • 别名：
    nusA; b3169; JW3138; Transcription termination/antitermination protein NusA; N utilization substance protein A; Transcription termination/antitermination L factor
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-495
  • 氨基酸序列
    MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. Participates in both transcription termination and antitermination. Involved in a variety of cellular termination and antitermination processes, such as Rho-dependent transcriptional termination and intrinsic termination. Domain AR2 interacts with a large number of other proteins and may serve as a platform to recruit these factors for transcriptional regulation. Involved in phage lambda N-mediated transcriptional antitermination. Also important for coordinating the cellular responses to DNA damage by coupling the processes of nucleotide excision repair and translesion synthesis to transcription.
  • 基因功能参考文献：
    1. The results suggest that interaction between NusA and NusG could play various regulatory roles during transcription, including recruitment of NusG to RNAP, resynchronization of transcription: translation coupling, and modulation of termination efficiency. PMID: 27174929
    2. The study showed that mutations in the NusA beta-flap domain specifically defective for N antitermination exhibited altered NusA-nascent RNA interaction and have widened RNA exit channel indicating an intricate role of flap domain in the antitermination. PMID: 25990722
    3. Data suggest that RNA exit channel duplexes interact specifically with RNA polymerase and NusA and compete with RfaH to control clamp position. PMID: 24275665
    4. Our results suggest that E. coli NusA can act as a protector to prevent protein aggregation under heat stress conditions in vitro and in the NusA-overexpressing strain. PMID: 23907089
    5. Studies suggest that NusA, in addition to its known roles in transcription elongation and termination, is important for coordinating the cellular responses to DNA damage. PMID: 21326909
    6. A missense nusA mutant xhibits a transcription termination-defective. PMID: 21602355
    7. Data suggest that that NusA participates in an alternative class of TCR involved in the identification and removal of a class of lesion. PMID: 20696893
    8. wild-type NusA function is required for stress-induced mutagenesis. This effect is unlikely to be due to defects in transcription of lac genes but rather is due to an inability to adapt and mutate in response to environmental stress. PMID: 20036541
    9. Structural model of the carboxy-terminal domain of the transcription factor Escherichia coli NusA and its interaction sites with the protein N of bacteriophage lambda, and the carboxyl terminus of the E. coli RNA polymerase alpha subunit. PMID: 15987884
    10. Titration experiments of the lambdaN(1-53)-nutBoxB RNA complex with NusA(339-495) revealed that ternary complex can be described in terms of two structurally independent binary interactions. PMID: 16584189
    11. NusA is required for lambda-related phage 82 antiterminator Q(82) to construct a stable complex in which RNA-based termination mechanisms have restricted access to the emerging transcript. PMID: 17889665
    12. findings show Rho termination, supported by NusA and NusG, is required to suppress the toxic activity of foreign genes PMID: 18487194
    13. NusA binds only the nut regions of spacer sequence between boxA and boxB. PMID: 19515940

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  • 亚细胞定位：
    Cytoplasm. Note=Colocalizes with nucleoids.
  • 蛋白家族：
    NusA family
  • 数据库链接：

    KEGG: ecj:JW3138

    STRING: 316385.ECDH10B_3343