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  4. Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP)

Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP)

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  • 中文名称：
    大肠杆菌phoP重组蛋白
  • 货号：
    CSB-MP326097ENV
  • 规格：
    ￥3960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP326097ENV could indicate that this peptide derived from Mammalian Cell-expressed Escherichia coli (strain K12) phoP.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP326097ENV could indicate that this peptide derived from Mammalian Cell-expressed Escherichia coli (strain K12) phoP.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    phoP
  • Uniprot No.：
  • 别名：
    phoP; b1130; JW1116; Transcriptional regulatory protein PhoP
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    Mammalian cell
  • 分子量：
    29.5kDa
  • 表达区域：
    1-223aa
  • 氨基酸序列
    MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHIPDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFELR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Just like other recombinant proteins, the production of this recombinant E.coli phoP protein began with appropriate cDNA and PCR methods, and then the phoP expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into Mammalian cell for the expression of the recombinant E.coli phoP protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 90%+.

    phoP is a gene providing an instruction of making a protein called transcriptional regulatory protein PhoP (also called Phop) widely presented in Gram-negative bacteria, such as Escherichia coli (E.coli). Phop is a virulence regulator conserved in both pathogenic and non-pathogenic Enterobacteriaceae. PhoP plays an important role as a transcriptional regulator in the two-component phoP/phoQ regulatory system with DNA-binding transcription activator activity. Moreover, This protein has phosphorelay response regulator activity and also can bind to transcription cis-regulatory region.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg(2+) environments and the control of acid resistance genes. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.
  • 基因功能参考文献：
    1. findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing PMID: 29183977
    2. Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation PMID: 23300478
    3. Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system. PMID: 22267510
    4. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competing with ribosome binding. PMID: 20345657
  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ecj:JW1116

    STRING: 316385.ECDH10B_1202