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  4. Recombinant Escherichia coli UPF0249 protein ChbG (chbG)

Recombinant Escherichia coli UPF0249 protein ChbG (chbG)

  • 货号：
    CSB-YP511543ENU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP511543ENU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP511543ENU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP511543ENU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP511543ENU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    chbG
  • Uniprot No.：
  • 别名：
    chbG; BWG_1546Chitooligosaccharide deacetylase; COD; EC 3.5.1.105; Chitin disaccharide deacetylase; Chitobiose deacetylase; Chitobiose-6P deacetylase; Chitotriose deacetylase; Chitotriose-6P deacetylase
  • 种属：
    Escherichia coli (strain K12 / MC4100 / BW2952)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-249
  • 氨基酸序列
    MERLLIVNAD DFGLSKGQNY GIIEACRNGI VTSTTALVNG QAIDHAVQLS RDEPSLAIGM HFVLTMGKPL TAMPGLTRDG VLGKWIWQLA EEDALPLEEI TQELVSQYLR FIELFGRKPT HLDSHHHVHM FPQIFPIVAR FAAEQGIALR ADRQMAFDLP VNLRTTQGFS SAFYGEEISE SLFLQVLDDA GHRGDRSLEV MCHPAFIDNT IRQSAYCFPR LTELDVLTSA SLKGAIAQRG YRLGSYRDV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the degradation of chitin. ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    YdjC deacetylase family, ChbG subfamily
  • 数据库链接：

    KEGG: ebw:BWG_1546