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  4. Recombinant Eukaryotic translation initiation factor 4E-3 (ife-3)

Recombinant Eukaryotic translation initiation factor 4E-3 (ife-3)

  • 中文名称：
    秀丽隐杆线虫ife-3重组蛋白
  • 货号：
    CSB-YP527673CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ife-3重组蛋白
  • 货号：
    CSB-EP527673CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ife-3重组蛋白
  • 货号：
    CSB-EP527673CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫ife-3重组蛋白
  • 货号：
    CSB-BP527673CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫ife-3重组蛋白
  • 货号：
    CSB-MP527673CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ife-3
  • Uniprot No.：
  • 别名：
    ife-3; B0348.6; Eukaryotic translation initiation factor 4E-3; eIF-4E-3; eIF4E-3; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-251
  • 氨基酸序列
    MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQKLQRR TQLLDHYWLE LLMAIVGEQF DEYGDYICGA VVNVRQKGDK VSLWTRDATR DDVNLRIGQV LKQKLSIPDT EILRYEVHKD SSARTSSTVK PRICLPAKDP APVKEKGPAA TTSPSNPGTE ATGTSPATPT P
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively. Within the pid-1 variant of the PETISCO complex binds to capped 21U-RNA precursor molecules, possibly playing a role in the processing of the 5' end of the molecules to promote binding of other complex components such as pid-3. However, it is not essential for the biogenesis of 21U-RNAs by itself. Within the tost-1 variant of the PETISCO complex binds to splice leader SL1 RNA fragments to possibly play a role in their processing.
  • 亚细胞定位：
    Cytoplasmic granule. Cytoplasm, perinuclear region.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 组织特异性：
    Highly expressed in the germline (at protein level).
  • 数据库链接：

    KEGG: cel:CELE_B0348.6

    STRING: 6239.B0348.6b.1

    UniGene: Cel.32382