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  4. Recombinant Gadus morhua Trypsin-1

Recombinant Gadus morhua Trypsin-1

  • 货号：
    CSB-YP018811GER
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018811GER-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018811GER
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018811GER
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    ; Trypsin-1; EC 3.4.21.4; Trypsin I
  • 种属：
    Gadus morhua (Atlantic cod)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-241
  • 氨基酸序列
    I VGGYECTKHS QAHQVSLNSG YHFCGGSLVS KDWVVSAAHC YKSVLRVRLG EHHIRVNEGT EQYISSSSVI RHPNYSSYNI NNDIMLIKLT KPATLNQYVH AVALPTECAA DATMCTVSGW GNTMSSVADG DKLQCLSLPI LSHADCANSY PGMITQSMFC AGYLEGGKDS CQGDSGGPVV CNGVLQGVVS WGYGCAERDH PGVYAKVCVL SGWVRDTMAN Y
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to get some informations for cod trypsin I.

A:
Thanks for your inquiry. This protein is available. The detail is as follow:
Recombinant Gadus morhua Trypsin-1
CSB-YP018811GER >> Yeast
CSB-EP018811GER >> E.coli
CSB-BP018811GER >> Baculovirus
CSB-MP018811GER >> Mammalian cell
Express Region:20-241aa ；mature full length protein.
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
Express Sequence:

IVGGYECTKHSQAHQVSLNSGYHFCGGSLVSKDWVVSAAHCYKSVLRVRLGEHHIRVNEGTEQYISSSSVIRHPNYSSYNINNDIMLIKLTKPATLNQYVHAVALPTECAADATMCTVSGWGNTMSSVADGDKLQCLSLPILSHADCANSYPGMITQSMFCAGYLEGGKDSCQGDSGGPVVCNGVLQGVVSWGYGCAERDHPGVYAKVCVLSGWVRDTMANY


Pls check if this protein can meet your request.
I'm afraid our protein are not GMP, HACCP certificated, but we have standard SOP and the good working habit. We are cooperation with a lot of pharmaceutical companies.
This protein is for research use only, can't be used for food.
Q:

The sequence is the wanted. I have some more questions and comments:
1.What is the activity of the trypsin e.g USP units / mg protein?
2.Can you supply large amount of active trypsin e.g in gram to kilogram scale?

A:
The answers are as follow:
1.What is the activity of the trypsin e.g USP units / mg protein? I'm afraid, we haven't test the activity of this protein.
2.Can you supply large amount of active trypsin e.g in gram to kilogram scale?
We have this ability, it depends on the protein request.

靶点详情

  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family