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  4. Recombinant Haplopelma schmidti Mu-theraphotoxin-Hh2a

Recombinant Haplopelma schmidti Mu-theraphotoxin-Hh2a

  • 货号：
    CSB-YP306061HUL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP306061HUL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP306061HUL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP306061HUL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP306061HUL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    ; Huwentoxin-IV; HwTx-IV; Huwentoxin-4; Huwentoxin-IVa; HWTX-IVa; Huwentoxin-IVb; HWTX-IVb; Huwentoxin-IVc; HWTX-IVc; Mu-theraphotoxin-Hs2a; Mu-TRTX-Hs2a
  • 种属：
    Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    53-87
  • 氨基酸序列
    ECLEIFKA CNPSNDQCCK SSKLVCSRKT RWCKYQI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This lethal neurotoxin (without cyclization at position 53) inhibits neuronal voltage-gated sodium channel Nav1.2/SCN2A (IC(50)=10-150 nM), rNav1.3/SCN3A (IC(50)=338 nM), Nav1.6/SCN8A (IC(50)=117 nM), and hNav1.7/SCN9A (IC(50)=9.6-33 nM). It inhibits activation of sodium channel by trapping the voltage sensor of domain II (DIIS4) in the closed configuration. The toxin neither shifts the Nav1.7/SCN9A activation curve nor modifies the slope factor. It does not slow fast-inactivation of hNav1.7/SCN9A channels. In addition, it has only a weak affinity for lipid membranes. This toxin also exists with a pyroglutamate at position 53. The sole difference observed between modified (mHwTx-IV) and unmodified toxins is that moderate or high depolarization voltages (200 mV) permit the unmodified toxin to dissociate, whereas mHwTx-IV toxin does not dissociate, even at high depolarization voltages. These data indicate that mHwTx-IV strongly binds to voltage sensor of sodium channel even at extreme depolarization voltages.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Huwentoxin-1 family, Htx-4 subfamily
  • 组织特异性：
    Expressed by the venom gland.