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  4. Recombinant Human 26S proteasome complex subunit DSS1 (SHFM1)

Recombinant Human 26S proteasome complex subunit DSS1 (SHFM1)

  • 中文名称：
    人SEM1重组蛋白
  • 货号：
    CSB-YP021263HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SEM1重组蛋白
  • 货号：
    CSB-EP021263HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SEM1重组蛋白
  • 货号：
    CSB-EP021263HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SEM1重组蛋白
  • 货号：
    CSB-BP021263HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SEM1重组蛋白
  • 货号：
    CSB-MP021263HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEM1
  • Uniprot No.：
  • 别名：
    SEM1; C7orf76; DSS1; SHFDG1; SHFM1; 26S proteasome complex subunit SEM1; 26S proteasome complex subunit DSS1; Deleted in split hand/split foot protein 1; Split hand/foot deleted protein 1; Split hand/foot malformation type 1 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-70
  • 氨基酸序列
    MSEKKQPVDL GLLEEDDEFE EFPAEDWAGL DEDEDAHVWE DNWDDDNVED DFSNQLRAEL EKHGYKMETS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.
  • 基因功能参考文献：
    1. Promoter methylation plays a role in modulating DSS1 gene expression. Promoter hypomethylation is a frequent event in melanoma and squamous cell carcinoma and is closely linked to poor prognosis. PMID: 27825810
    2. DSS1 is a multifunctional and intrinsically disordered protein. (Review) PMID: 26944332
    3. three phenotypic subregions within the SHFM1 locus link both size and genomic position of the chromosomal aberrations to the clinical variability seen in SHFM1 patients PMID: 26839112
    4. SHFM1 confers cell cycle progression and resistance to p53 stabilizing drugs in gastric cancer cells. PMID: 25697906
    5. by targeting RPA and mimicking DNA, DSS1 functions with BRCA2 in a two-component homologous recombination mediator complex in genome maintenance and tumor suppression PMID: 26145171
    6. Breast cancers with high DSS1 expression have worse prognosis and shorter relapse-free survival times. PMID: 24289229
    7. Mutation screening of the SHFM1 gene in familial breast/ovarian cancer cases. PMID: 23371468
    8. DSS1 protein is critically involved in the maintenance of the transformed phenotype in cervical cancer cells, and that it might be a specific, robust and reliable marker for early detection, diagnosis and trea PMID: 23024267
    9. DSS1 has a role in homologous recombinational repair in human cells PMID: 20817001
    10. p63 binds to an enhancer element in the SHFM1 locus and this element controls expression of DLX6 and DLX5 which are important for limb development. PMID: 20808887
    11. The DSS1 c.143G>A variant is associated with reduced DSS1 expression at RNA and protein levels and altered traffic of the DSS1 protein from the cytoplasm to the nucleus. These alterations could impair DSS1 function and may be implicated in skin cancer. PMID: 20220765
    12. 3.1 angstrom crystal structure of approximately 90-kilodalton BRCA2 domain bound to DSS1, which reveals three oligonucleotide-binding (OB) folds and a helix-turn-helix (HTH) motif PMID: 12228710
    13. DSS1, responsible for autism, was studied in a linkage disequilibrium model. PMID: 17406092
    14. DSS1 has been shown to interact with components of the 26S proteasome in Saccharomyces cerevisiae and in human tumour cells PMID: 17563742
    15. Data suggest that the R3IM motif of DSS1, in conjunction with the complexes of 19S RP and 20S core particle, regulates proteasome interaction through RPN3/S3 molecule, and utilizes a specific subset of poly-ubiquitinated p53 as a substrate. PMID: 18775730

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DSS1/SEM1 family
  • 组织特异性：
    Expressed in limb bud, craniofacial primordia and skin.
  • 数据库链接：

    HGNC: 10845

    OMIM: 601285

    STRING: 9606.ENSP00000248566

    UniGene: Hs.489201