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  4. Recombinant Human ADP-ribosylation factor-binding protein GGA3 (GGA3)

Recombinant Human ADP-ribosylation factor-binding protein GGA3 (GGA3)

  • 中文名称：
    人GGA3重组蛋白
  • 货号：
    CSB-YP882151HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GGA3重组蛋白
  • 货号：
    CSB-EP882151HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GGA3重组蛋白
  • 货号：
    CSB-EP882151HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GGA3重组蛋白
  • 货号：
    CSB-BP882151HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GGA3重组蛋白
  • 货号：
    CSB-MP882151HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GGA3
  • Uniprot No.：
  • 别名：
    ADP ribosylation factor binding protein 3; ADP ribosylation factor binding protein GGA 3; ADP ribosylation factor binding protein GGA3 ; ADP-ribosylation factor-binding protein GGA3; ARF binding protein GGA 3; ARF binding protein GGA3; ARF-binding protein 3; gamma ear-containing; GGA 3; GGA3; GGA3_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 3; Golgi localized gamma ear containing ARF binding protein 3; Golgi-localized; KIAA0154
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-723
  • 氨基酸序列
    MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL AHKIQSPQEW EALQALTVLE ACMKNCGRRF HNEVGKFRFL NELIKVVSPK YLGDRVSEKV KTKVIELLYS WTMALPEEAK IKDAYHMLKR QGIVQSDPPI PVDRTLIPSP PPRPKNPVFD DEEKSKLLAK LLKSKNPDDL QEANKLIKSM VKEDEARIQK VTKRLHTLEE VNNNVRLLSE MLLHYSQEDS SDGDRELMKE LFDQCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ VINGEVATLT LPDSEGNSQC SNQGTLIDLA ELDTTNSLSS VLAPAPTPPS SGIPILPPPP QASGPPRSRS SSQAEATLGP SSTSNALSWL DEELLCLGLA DPAPNVPPKE SAGNSQWHLL QREQSDLDFF SPRPGTAACG ASDAPLLQPS APSSSSSQAP LPPPFPAPVV PASVPAPSAG SSLFSTGVAP ALAPKVEPAV PGHHGLALGN SALHHLDALD QLLEEAKVTS GLVKPTTSPL IPTTTPARPL LPFSTGPGSP LFQPLSFQSQ GSPPKGPELS LASIHVPLES IKPSSALPVT AYDKNGFRIL FHFAKECPPG RPDVLVVVVS MLNTAPLPVK SIVLQAAVPK SMKVKLQPPS GTELSPFSPI QPPAAITQVM LLANPLKEKV RLRYKLTFAL GEQLSTEVGE VDQFPPVEQW GNL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane.
  • 基因功能参考文献：
    1. In the absence of GGA3, integrins are increasingly retained inside the cell, traffic toward the perinuclear lysosomal compartment and their degradation is enhanced. PMID: 26935970
    2. This work identifies GGA3 as a key player in a novel DXXLL-mediated endosomal sorting machinery that targets TrkA to the plasma membrane, where it prolongs the activation of Akt signaling and survival responses. PMID: 26446845
    3. role of GGA3 in the cell surface export of alpha2B-adrenergic receptor PMID: 26811329
    4. GGA3 is involved in the cellular processes relevant for Alzheimer's disease pathogenesis. PMID: 23970038
    5. Subcellular fractionation of Alzheimer's disease cortex with low levels of Golgi-localized GGA proteins shows an alteration of beta-secretase distribution and extensive co-localization with amyloid beta precursor protein APP. PMID: 21440067
    6. GGA3 tightly and inversely regulate BACE1 levels via interaction with ubiquitin sorting machinery PMID: 20484053
    7. structures of the VHS domain of human GGA3 complexed with signals from both mannose-6-phosphate receptors PMID: 11859375
    8. These results indicate that GGA3-S is predominantly expressed in human tissues except the brain and in cell lines. PMID: 12810073
    9. RNAi of GGA3 expression results in accumulation of the cation-independent mannose 6-phosphate receptor and internalized epidermal growth factor (EGF) within enlarged early endosomes. This perturbation impairs the degradation of internalized EGF. PMID: 15039775
    10. found that the human GGA3 protein GAT domain contains two ubiquitin binding motifs that bind to the same surface of ubiquitin PMID: 15494413
    11. GGA proteins funstion with the phosphorylated ACDL in the memasin 2-recycling pathway from endosomes to trans Golgi on the way back to the cell surface. PMID: 15615712
    12. crystal structure of the GAT domain PMID: 15701688
    13. GGA3, becomes transiently phosphorylated upon activation of the epidermal growth factor (EGF) receptor. (GGA3) PMID: 16135791
    14. CK2-activated phosphorylation cascade controlling PACS-1- and GGA3-mediated CI-MPR sorting, is reported. PMID: 16977309
    15. ubiquitin binding and ubiquitylation of GGA3-GAT domain are mutually inseparable through a ubiquitin ligase activity of hVPS18 PMID: 16996030
    16. We have elucidated a GGA3-dependent mechanism regulating BACE levels and beta-secretase activity. This mechanism may explain increased cerebral levels of BACE and Abeta following cerebral ischemia and existing in AD. PMID: 17553422
    17. seladin-1 downregulation increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis PMID: 19815556

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  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    GGA protein family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 17079

    OMIM: 606006

    KEGG: hsa:23163

    STRING: 9606.ENSP00000245541

    UniGene: Hs.87726