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  4. Recombinant Human ATP-dependent DNA helicase PIF1 (PIF1)

Recombinant Human ATP-dependent DNA helicase PIF1 (PIF1)

  • 货号：
    CSB-YP863987HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP863987HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP863987HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP863987HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP863987HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIF1
  • Uniprot No.：
  • 别名：
    ATP dependent DNA helicase PIF1; ATP-dependent DNA helicase PIF1; C15orf20; DNA helicase homolog PIF1; DNA helicase PIF1; DNA repair and recombination helicase PIF1; Petite integration frequency 1; PIF; PIF1 5' to 3' DNA helicase; PIF1 5' to 3' DNA helicase homolog; PIF1; PIF1 DNA helicase; PIF1/RRM3 DNA helicase like protein; PIF1/RRM3 DNA helicase-like protein; PIF1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-641
  • 氨基酸序列
    MLSGIEAAAG EYEDSELRCR VAVEELSPGG QPRRRQALRT AELSLGRNER RELMLRLQAP GPAGRPRCFP LRAARLFTRF AEAGRSTLRL PAHDTPGAGA VQLLLSDCPP DRLRRFLRTL RLKLAAAPGP GPASARAQLL GPRPRDFVTI SPVQPEERRL RAATRVPDTT LVKRPVEPQA GAEPSTEAPR WPLPVKRLSL PSTKPQLSEE QAAVLRAVLK GQSIFFTGSA GTGKSYLLKR ILGSLPPTGT VATASTGVAA CHIGGTTLHA FAGIGSGQAP LAQCVALAQR PGVRQGWLNC QRLVIDEISM VEADLFDKLE AVARAVRQQN KPFGGIQLII CGDFLQLPPV TKGSQPPRFC FQSKSWKRCV PVTLELTKVW RQADQTFISL LQAVRLGRCS DEVTRQLQAT ASHKVGRDGI VATRLCTHQD DVALTNERRL QELPGKVHRF EAMDSNPELA STLDAQCPVS QLLQLKLGAQ VMLVKNLSVS RGLVNGARGV VVGFEAEGRG LPQVRFLCGV TEVIHADRWT VQATGGQLLS RQQLPLQLAW AMSIHKSQGM TLDCVEISLG RVFASGQAYV ALSRARSLQG LRVLDFDPMA VRCDPRVLHF YATLRRGRSL SLESPDDDEA ASDQENMDPI L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.
  • 基因功能参考文献：
    1. Structural and functional characterization of the unwinding mechanism of Bacteroides and human Pif1 helicases has been reported. PMID: 26904952
    2. s characterised a functional role for human PIF1 in DNA replication that becomes important for cell growth under oncogenic stress. PMID: 25359767
    3. Pif1 readily unfolds a parallel quadruplex DNA substrate in a multiturnover reaction and also generates some product under single cycle conditions PMID: 25589786
    4. A minimum of 10 oligonucleotide bases are required for PIF1 binding and the hydrolysis of ATP. PMID: 23924759
    5. the yeast ortholog of Pif1 appears to move along DNA in single nucleotide or base pair steps, powered by hydrolysis of 1 molecule of ATP. PMID: 23596008
    6. Evidence that the PIF1 N-terminal (PINT) domain as crucial functions in PIF1 helicase. PMID: 18835853
    7. when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening PMID: 23657261
    8. PIF1 variant L319P was identified in three breast cancer families PMID: 22347400
    9. Findings suggest roles for PIF1 in S-phase entry and progression that are essential to protect human tumor cells from apoptosis. PMID: 21616935
    10. Human Pif1 could have a role in processing G4 structures that arise in the single-stranded nucleic acid intermediates formed during DNA replication and gene expression. PMID: 20524933
    11. hPif1 may regulate telomere elongation by decreasing telomerase processivity, possibly via a mechanism that involves the unwinding of telomerase RNA from telomeric DNA by hPif1. PMID: 16522649
    12. Human PIF, like S. cerevisiae Pif1p, plays a role in telomerase regulation. PMID: 17172855
    13. hPif1 in the nucleus may be involved in chromosome maintenance in association with DNA replication PMID: 17827721
    14. hPif1 specifically recognizes and unwinds DNA structures resembling putative stalled replication forks. PMID: 19700773

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  • 亚细胞定位：
    Nucleus.; [Isoform 4]: Mitochondrion.
  • 蛋白家族：
    Helicase family, PIF1 subfamily
  • 组织特异性：
    Weak ubiquitous expression.
  • 数据库链接：

    HGNC: 26220

    OMIM: 610953

    KEGG: hsa:80119

    STRING: 9606.ENSP00000268043

    UniGene: Hs.112160