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  4. Recombinant Human ATPase ASNA1 (ASNA1)

Recombinant Human ATPase ASNA1 (ASNA1)

  • 货号：
    CSB-EP002218HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ASNA1
  • Uniprot No.：
  • 别名：
    ARSA 1; ARSA; ArsA arsenite transporter ATP binding homolog 1; ArsA arsenite transporter; ATP binding; E. coli; homolog of; 1; ArsA arsenite transporter; ATP-binding; homolog 1 (bacterial); ARSA I; ARSA-I; ARSA1; ARSAI; Arsenical pump driving ATPase; Arsenical pump-driving ATPase; Arsenical resistance ATPase; Arsenite translocating ATPase; Arsenite transporting ATPase; Arsenite-stimulated ATPase; ASNA 1; ASNA I; ASNA-I; ASNA_HUMAN; ASNA1; Asna1 protein ; ASNAI; ATPase ASNA1; GET3; Golgi to ER traffic 3 homolog; hARSA-I; hASNA-I; MGC3821; Transmembrane domain recognition complex 40 kDa ATPase subunit; Transmembrane domain recognition complex; 40kDa; TRC40
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    54.7kDa
  • 表达区域：
    2-348aa
  • 氨基酸序列
    AAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.
  • 基因功能参考文献：
    1. The s conclude that the TRC40 pathway is critical for hearing and propose that otoferlin is an essential substrate of this pathway in hair cells. PMID: 27458190
    2. Asna1/TRC40 is required at a late step of herpes simplex virus type 1 infection for efficient release of mature virions to the extracellular milieu. PMID: 27765046
    3. reveal unanticipated complexity in the mutual regulation of the TRC40 receptor subunits and raise the question as to the role of the excess CAML in the endoplasmic reticulum PMID: 27226539
    4. Emerin interacts with TRC40 in situ. PMID: 26675233
    5. The repertoire of VAPB interactors is more diverse than previously anticipated and link VAPB to the function of ATPase complexes such as p97/FAF1 and ASNA1/transmembrane-domain recognition complex. PMID: 24885147
    6. Proteins bind to TRC40 and can utilise this component for their delivery to the ER membrane. PMID: 22505607
    7. PEX19 formed a complex with the peroxisomal tail anchored protein PEX26 in the cytosol and translocated it directly to peroxisomes by a TRC40-independent class I pathway. PMID: 23460677
    8. Results indicate calcium-modulating cyclophilin ligand (CAML) and WRB as components of the TRC40 receptor complex and a crucial mechanism for driving ER membrane insertion of TA proteins in mammalian cells. PMID: 23041287
    9. Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone. PMID: 21878631
    10. The coiled-coil domain of WRB is the binding site for TRC40/Asna1. PMID: 21444755
    11. Asna1 is required for efficient histocompatibility (HLA) class I downregulation mediated by viral protein BNLF2a. PMID: 21296983
    12. Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the endoplasmic reticulum membrane. PMID: 20375064
    13. Human ASNA1 is highly expressed in pancreatic beta cells, but not in other pancreatic endocrine cell types, and regulates insulin secretion in cultured cells. PMID: 17289575
    14. TRC40/Asna-1 interacts posttranslationally with tail-anchored proteins in a transmembrane domain-dependent manner for delivery to a proteinaceous receptor at the endoplasmic reticulum membrane. PMID: 17382883
    15. the hydrophobicity of the TA region dictates whether a precursor is delivered to the ER via the Hsp40/Hsc70 or Asna-1/TRC40-dependent route. PMID: 18667436
    16. ASNA1 is a target to overcome platinum resistance in ovarian cancer PMID: 19724867

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.
  • 蛋白家族：
    ArsA ATPase family
  • 组织特异性：
    Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.
  • 数据库链接：

    HGNC: 752

    OMIM: 601913

    KEGG: hsa:439

    STRING: 9606.ENSP00000349887

    UniGene: Hs.465985