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  4. Recombinant Human Acid-sensing ion channel 1 (ASIC1), partial

Recombinant Human Acid-sensing ion channel 1 (ASIC1), partial

  • 货号：
    CSB-YP001142HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001142HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001142HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001142HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001142HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ASIC1
  • Uniprot No.：
  • 别名：
    ACCN2; ACCN2_HUMAN; Acid sensing ion channel 1; Acid sensing ion channel 1a protein; Acid sensing ion channel; Acid-sensing ion channel 1; AI843610; Amiloride sensitive cation channel 2 neuronal; Amiloride-sensitive cation channel 2; ASIC ; ASIC1; ASIC1A; B530003N02Rik; BNaC2; Brain sodium channel 2; Cation channel amiloride sensitive neuronal 2; hBNaC2; neuronal
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.; Isoform 1 does not display proton-gated cation channel activity.
  • 基因功能参考文献：
    1. Data show that acid-sensing ion channel 1a (ASIC1a) expression level was significantly higher in gastric carcinoma (GC) tissues. PMID: 29584803
    2. Study shows that bile acids potentiate proton-activated currents in Xenopus laevis oocytes expressing human acid-sensing ion channel. Site-directed mutagenesis demonstrated that the amino acid residue G433 is critically involved in the potentiating effect of bile acids on ASIC1a activation by protons. PMID: 28193786
    3. Genetic variants in the ACCN2 are associated with differential sensitivity to CO2. PMID: 28121219
    4. The deactivation of ASIC1 was steeply dependent on the pH, spanning nearly three orders of magnitude from extremely fast at pH 8 to very slow at pH 7. PMID: 28265090
    5. Results show that the "acidic pocket," the binding site of several toxins, is not essential for channel function but has, rather, a modulatory role. Furthermore, the study describes the structural rearrangements occurring in this domain during ASIC activity, and highlight the importance of the "palm" domain in channel opening and current decay. PMID: 28320963
    6. our results suggest that ASIC1a is critical for liver cancer cell proliferation and its deregulation is frequently linked to liver cancer PMID: 27462920
    7. the data demonstrate a functional link between ASICs and [Ca(2+)]i/RhoA pathway, which contributes to the acidity-induced epithelial-mesenchymal transition. PMID: 28518134
    8. ASIC1 contributes to breast cancer pathogenesis in response to acidic tumor microenvironments PMID: 26686084
    9. Data show that acid sensing ion channel 1a (ASIC1a) mediated calcium influx, which resulted in the activation of PI3K/AKT signaling and increased drug resistance, suggesting that ASIC1a/Ca(2+)/PI3K/AKT signaling represents a pathway that regulates drug resistance, thus offering a potential target for chemotherapy of hepatocellular carcinoma (HCC). PMID: 27918554
    10. Downregulation of ASIC1 inhibits gastric cancer growth via decreasing autophagy, therefore this strongly suggests a therapeutic role for ASIC1 in gastric cancer. PMID: 28111260
    11. The C mutated allele for ACCN2 rs685012 polymorphism was more frequent in panic disorder patients. The ACCN2 TT/ADORA2A CT diplotype were more represented in controls. This suggests a role for ACCN2 rs685012 polymorphism in PD development in Caucasian PMID: 26589317
    12. analysis of a major ASIC1a homotetramer at the surface membrane of the cell expressing functional ASIC1a channel PMID: 26252376
    13. Our findings support previously reported associations between ASIC1 and panic/anxiety, but not other genes previously associated with anxiety disorders. The PMID: 25913301
    14. Putative GABAA and ASIC1a channels functionally interact with each other, possibly via an inter-molecular association by forming a novel protein complex. PMID: 24923912
    15. A novel interaction between ASIC1 and integrin-Beta1, mediated by alpha-actinin was determined in glioma tumor cells. PMID: 26108662
    16. Suppression of ASIC1alpha expression by RNAi attenuated the malignant phenotype of hepatocellular carcinoma cells, suggesting a novel approach for anticancer gene therapy. PMID: 25613068
    17. ASIC1a opening is accompanied by a distance increase between adjacent thumb and palm domains as well as a movement of Glu-235 relative to the knuckle helix. PMID: 26070563
    18. ASIC1A and ENaCalpha form functional heterotrimers acting as ion channels. PMID: 26032502
    19. This study suggests that ASIC1 may play a role as mediators of inflammatory pain and be involved in the pathogenesis of frozen shoulder. PMID: 26033064
    20. Genetic variation at ACCN2 appears to be associated with panic disorder and with amygdala phenotypes that have been linked to proneness to anxiety. PMID: 24529281
    21. Down-regulated expression of ASIC1 RNA and protein was detected in the dysplastic cortex of focal cortical dysplasia patients. PMID: 24682892
    22. Provide evidence for the roles of OGR1 and ASIC1a in the regulation of intestinal passive Mg(2+) absorption. PMID: 24375028
    23. results suggest that FPI induces cerebral acidosis that activates ASIC channels and contributes to secondary injury in TBI. They also suggest a therapeutic strategy to attenuate the adverse consequences of TBI PMID: 23991103
    24. it can be concluded that CaMKII regulates the activity of ASIC1, which is associated with the ability of GBM cells to migrate. PMID: 24100685
    25. Regions of the extracellular palm domain and the beta(11-12) linker are important for inactivation and steady-state desensitization of ASIC1a. PMID: 23977127
    26. ASIC1a channels desensitize rapidly in the presence of a continuous acidosis or following a preexposure to minor pH drop, raising doubt for their contributions to the acidosis-mediated neuronal injury. PMID: 23224900
    27. ASIC1a has two desensitized states with markedly different stabilities. PMID: 23048040
    28. The results of this study demonstrated for the first time an association between an ASC1a variant allele and TLE in a Han Chinese population. PMID: 21664108
    29. These data suggest that different ENaC/ASIC1 subunits interact and could combine to form a hybrid channel that likely underlies the amiloride-sensitive current seen in human glioma cells. PMID: 21346156
    30. Results indicate that PICK1 regulates trafficking and function of ASIC1a in a lipid binding-dependent manner. PMID: 21176140
    31. analysis of the ASIC1a and ASIC1b calcium permeable human acid-sensing ion channel 1 transcript variants PMID: 21036899
    32. analysis of a complex between the sigma-1 receptor and a target ion channel, analysis of the stoichiometry of the interaction as 1 sigma-1 receptor/1 ASIC1a subunit PMID: 20371317
    33. matriptase is expressed in glioma cells and that matriptase specifically cleaves ASIC1 in heterologous expression systems. PMID: 20601429
    34. functional homomeric ASIC1a channels are predominantly expressed in neurons from the human brain. PMID: 20216553
    35. A combined computational and functional approach identifies new residues involved in pH-dependent gating of ASIC1a. PMID: 20299463
    36. The contact region between three domains of the extracellular loop of ASIC1a is critical for channel function. PMID: 20215117
    37. analysis of amiloride docking to acid-sensing ion channel-1 PMID: 20048170
    38. wild type CFTR elevates the acid-gated Na(+) current of ASIC1a/2a in part by altering the kinetics of extracellular Na(+) interaction. PMID: 11748227
    39. participation of multiple PKC isotypes contributes to the overall activity of BNaC2 PMID: 12244121
    40. ASICs are leading acid sensors in human nociceptors and VR1 participates in the nociception mainly under extremely acidic conditions. PMID: 12393854
    41. ASIC1a and H(+)-gated currents may contribute to the development of abnormal fear and to anxiety disorders. PMID: 14988500
    42. Ibuprofen studies show ASIC1a is a significant contributor to cutaneous acid-induced pain. PMID: 15574747
    43. These observations strongly suggest the acid-induced current is mediated by ASIC1 channels. PMID: 15576453
    44. expression of vanilloid receptor subtype-1 and acid-sensing ion channel genes in the human trigeminal ganglion neurons PMID: 15738111
    45. Data found ACCN2 interact with ataxin-3 and provide new clues for the pathogenesis of spinocerebellar ataxia type 3 and Machado-Joseph disease (SCA3/MJD). PMID: 15952105
    46. functional expression of ASICs is characteristic feature of adenoid cystic carcinomas cells PMID: 17324378
    47. Sulfhydryl compounds potentiate H(+)-gated currents via two mechanisms, metal chelation and redox modulation of target amino acids. PMID: 17392378
    48. Spontaneous currents represent activation of acid-sensitive ion channels (ASICs) by autocrine vesicular release of protons from HEK cells. PMID: 17443677
    49. endogenous peptides shift steady-state desensitization suggests that RF-amides could impact the role of ASIC1a in both pain and neuronal damage following stroke and ischemia. PMID: 17984098
    50. A case-control twin study could not detect association of ACCN2 with genetic risk shared among human anxiety spectrum disorders. PMID: 18349698

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, ASIC1 subfamily
  • 组织特异性：
    Expressed in most or all neurons.
  • 数据库链接：

    HGNC: 100

    OMIM: 602866

    KEGG: hsa:41

    UniGene: Hs.274361