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  4. Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A)

Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A)

  • 中文名称：
    Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A(ANP32A),Yeast
  • 货号：
    CSB-YP001822HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A(ANP32A),Yeast
  • 货号：
    CSB-EP001822HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A(ANP32A),Yeast
  • 货号：
    CSB-EP001822HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A(ANP32A),Yeast
  • 货号：
    CSB-BP001822HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member A(ANP32A),Yeast
  • 货号：
    CSB-MP001822HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ANP32A
  • Uniprot No.：
  • 别名：
    acidic (leucine-rich) nuclear phosphoprotein 32 family; member A; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; AN32A_HUMAN; ANP32A; C15orf1; Cerebellar leucine rich acidic nuclear protein; Hepatopoietin Cn; HPPCn; I1PP2A; Inhibitor 1 of protein phosphatase 2A; inhibitor-1 of protein phosphatase-2A; Lanp; Leucine rich acidic nuclear protein; Leucine-rich acidic nuclear protein; MAPM; Mapmodulin; MGC119787; MGC150373; PHAPI; Potent heat stable protein phosphatase 2A inhibitor I1PP2A; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; Putative HLA DR associated protein I; Putative HLA-DR-associated protein I; Putative human HLA class II associated protein I; Putative human HLA class II-associated protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-249
  • 氨基酸序列
    MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription. Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation. In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking. Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition. Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A.; (Microbial infection) Plays an essential role in influenza A, B and C viral genome replication. Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region. Plays also an essential role in foamy virus mRNA export from the nucleus.
  • 基因功能参考文献：
    1. our study reveals that ANP32A dysregulation may be a critical factor contributing to acute megakaryoblastic leukemia PMID: 29269781
    2. These data suggest compensatory mechanisms underlying viral Polymerase adaptation to host ANP32A independent of species-specific interactions. PMID: 28903035
    3. ANP32A promoted CRC proliferation by inhibition of p38. PMID: 28731192
    4. Data show that PP32 and SET/TAF-Ibeta proteins block HAT1-mediated H4 acetylation. PMID: 28977641
    5. Results suggest that ANP32A is commonly increased in oral squamous cell carcinoma and ANP32A protein could act as a potential biomarker for prognosis assessment of oral cancer patients with lymph node metastasis. PMID: 26918356
    6. According to the cellular function of the characterized targets of ProTalpha, and the evolution in the composition of the diverse ProTalpha-complexes when proliferation activity was reduced or apoptosis induced, leads to hypothesized that ProTalpha interactions might be related to the proliferation activity and control of the cell survival. PMID: 29106904
    7. Host-derived proteins pp32 and APRIL interact with a free form of influenza virus RNA-dependent RNA polymerase and preferentially upregulates viral RNA synthesis rather than cRNA synthesis. PMID: 26512887
    8. High-resolution crystal structure of the leucine-rich repeat domain of the human tumour suppressor PP32A (ANP32A) has been reported. PMID: 26057796
    9. ANP32A represents an essential host partner co-opted to support influenza virus replication and is a candidate host target for novel antivirals PMID: 26738596
    10. conformational analysis of the C-terminal transition state of the leucine-rich repeat domain of PP32 PMID: 25902505
    11. Demonstrate that HPPCn attenuated oxidative injury and fibrosis induced by ethanol feeding and that the SphK1/S1P/S1PRs signalling pathway contributes to this protective effect. PMID: 23839903
    12. The HPPCn expression might be involved in the development of hepatocellular carcinoma and could be served as a promising biomarker. PMID: 20683644
    13. MicroRNA-21 targets tumor suppressor genes ANP32A and SMARCA4. PMID: 21317927
    14. pp32 interacted with STAT1 and STAT2 in an IFNB-dependent manner. PMID: 21325029
    15. Data provide evidence that the tumor suppressor function of pp32 can be attributed to its ability to disrupt HuR binding to target mRNAs encoding key proteins for cancer cell survival and drug efficacy. PMID: 21152064
    16. Sphingosine interaction with acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) regulates PP2A activity and cyclooxygenase (COX)-2 expression in human endothelial cells. PMID: 20558741
    17. Regulation of histone acetylation and transcription by nuclear protein pp32, a subunit of the INHAT complex. PMID: 11830591
    18. regulatory roles of oncoprotein ProT and tumor suppressor PHAP in apoptosis PMID: 12522243
    19. LANP could play a key role in neuronal development and/or neurodegeneration by its interactions with microtubule associated proteins PMID: 12807913
    20. Set/TAF-Ibeta and pp32 proteins have roles as transducers of chromatin signaling by integrating chromatin hypoacetylation and transcriptional repression PMID: 15136563
    21. tyrosine phosphorylation of PHAPI is accompanied by the release of PP2A from association with PHAPI, allowing increased phosphatase activity of PP2A and consequent complete dephosphorylation of the ERK kinase, MEK1/2, by 10 min and of ERK1/2 by 60 min PMID: 15247276
    22. We now show pp32 increases androgen receptor-mediated transcription and the retinoblastoma protein modulates this activity. PMID: 16009334
    23. The ANP32A protein is characterizing as modulators of Wnt protein signaling of Axin-1. PMID: 16169070
    24. pp32 plays a repressive role by inhibiting transcription and triggering apoptosis. PMID: 16341127
    25. Immunoblot detection revealed that the inhibitor I1PP2A is expressed throughout the brain, including the hippocampus, temporal cortex, parietal cortex, subcortical nuclei and brain stem. PMID: 17266954
    26. LANP and ATAXN1 interact in E4F-mediated transcriptional repression. PMID: 17557114
    27. These results identify pp32/PHAPI as regulator of the apoptosis response of cancer cells in vitro and in vivo, and as a predictor of survival following chemotherapy for advanced non-small-cell lung cancer. PMID: 17962813
    28. Results describe the solution structure of the evolutionarily conserved N-terminal leucine-rich repeat (LRR) domain of Anp32a and model its interactions with other proteins. PMID: 18410380
    29. PHAPI, CAS, and Hsp70 function together to accelerate nucleotide exchange on Apaf-1 and prevent inactive Apaf-1/cytochrome c aggregation. PMID: 18439902
    30. PHAPI/pp32 suppresses tumorigenesis by stimulating apoptosis. PMID: 19121999
    31. ANP32A is involved in the pathogenesis of osteoarthritis of the hip. PMID: 19565487

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Endoplasmic reticulum.
  • 蛋白家族：
    ANP32 family
  • 组织特异性：
    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus cal
  • 数据库链接：

    HGNC: 13233

    OMIM: 600832

    KEGG: hsa:8125

    STRING: 9606.ENSP00000417864

    UniGene: Hs.458747