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  4. Recombinant Human Adapter protein CIKS (TRAF3IP2)

Recombinant Human Adapter protein CIKS (TRAF3IP2)

  • 货号：
    CSB-YP024150HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024150HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024150HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024150HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024150HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Act 1; ACT1; Activator of cAMP responsive element modulator (CREM) in testis; Adapter protein CIKS; C6ORF4; C6ORF5; C6ORF6; Chromosome 6 Open Reading Frame 4; Chromosome 6 Open Reading Frame 5; Chromosome 6 Open Reading Frame 6; CIKS_HUMAN; Connection to IKK and SAPK / JNK; Connection to IKK and SAPK/JNK; Nuclear Factor Kappa B Activator 1; Nuclear factor NF kappa B activator 1; Nuclear factor NF-kappa-B activator 1; TRAF3 interacting protein 2; TRAF3-interacting protein 2; TRAF31P2; TRAF3IP2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-574
  • 氨基酸序列
    MPPQLQETRM NRSIPVEVDE SEPYPSQLLK PIPEYSPEEE SEPPAPNIRN MAPNSLSAPT MLHNSSGDFS QAHSTLKLAN HQRPVSRQVT CLRTQVLEDS EDSFCRRHPG LGKAFPSGCS AVSEPASESV VGALPAEHQF SFMEKRNQWL VSQLSAASPD TGHDSDKSDQ SLPNASADSL GGSQEMVQRP QPHRNRAGLD LPTIDTGYDS QPQDVLGIRQ LERPLPLTSV CYPQDLPRPL RSREFPQFEP QRYPACAQML PPNLSPHAPW NYHYHCPGSP DHQVPYGHDY PRAAYQQVIQ PALPGQPLPG ASVRGLHPVQ KVILNYPSPW DHEERPAQRD CSFPGLPRHQ DQPHHQPPNR AGAPGESLEC PAELRPQVPQ PPSPAAVPRP PSNPPARGTL KTSNLPEELR KVFITYSMDT AMEVVKFVNF LLVNGFQTAI DIFEDRIRGI DIIKWMERYL RDKTVMIIVA ISPKYKQDVE GAESQLDEDE HGLHTKYIHR MMQIEFIKQG SMNFRFIPVL FPNAKKEHVP TWLQNTHVYS WPKNKKNILL RLLREEEYVA PPRGPLPTLQ VVPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling. Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein. Essential adapter molecule in IL17A-mediated signaling. Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways.
  • 基因功能参考文献：
    1. AP1 binding sites were enriched upstream of genes up-regulated by TRAF3IP2 silencing. Correspondingly, nuclear expression of FosB and Fra1 was increased in TRAF3IP2-silenced cells. Many genes involved in host defense were induced by IL-17 in a TRAF3IP2-dependent fashion. PMID: 28274739
    2. TRAF3IP2 SNP rs33980500 associated with no achievement of low disease activity nor remission at 6 months PMID: 28107378
    3. the first report to describe a non-adaptor function of Act1 by directly binding to the promoter region of IL-17A responsive genes and directly regulate their transcription. PMID: 27723765
    4. ACT1 is an essential adaptor molecule of Il-17-induced expression of inflammation-related gene targets. PMID: 27660002
    5. Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID: 25908521
    6. A G/G genotype of rs766748 in IL-17F, and a C/C or C/A genotype of rs1883136 in TRAF3IP2. PMID: 26558270
    7. The suppressive effects of miR-30a were mediated by directly targeting Traf3ip2 mRNA PMID: 26376209
    8. TRAF3IP2 may play a causal role in aldosterone-induced adverse cardiac remodeling in vivo. PMID: 26148936
    9. Single nucleotide polymorphisms in RBPJ, IL1R1, REV3L, TRAF3IP2, IRF1 and ICOS showed association with rheumatoid arthritis in black South Africans. PMID: 25014791
    10. A variant (rs76228616 SNP) in TRAF3IP2 gene could be involved in susceptibility to Steven-Johnson Syndrome. PMID: 25775161
    11. identify Syk as an upstream signaling molecule in IL-17A-induced Act1-TRAF6 interaction in keratinocytes, and inhibition of Syk can attenuate CCL20 production PMID: 25202827
    12. These results indicate that oxLDL-induced endothelial cell death and dysfunction are mediated via TRAF3IP2 and that native HDL3 and the AMPK activators inhibit this response. PMID: 24561578
    13. Replicate the association of TRAF3IP2-_rs33980500 variant with the susceptibility to psoriasis. PMID: 24005976
    14. this study demonstrated that although ACT1-v2-D10N is nonfunctional, ACT1-v1-D19N retains the ability to interact with Hsp90 and is fully responsive to IL-17A stimulation. PMID: 25024377
    15. Genetic polymorphism in the TRAF3IP2 gene is associated with psoriasis vulgaris in a Japanese population. PMID: 24373565
    16. This study provides evidence that TRAF5 and TRAF3IP2 genes are involved in the development ofBehcet's disease (BD) and Vogt-Koyanagi-Harada (VKH) syndrome. PMID: 24416204
    17. Results suggest that Act1 might play a key role in the pathophysiology and the treatment of rheumatoid arthritis. PMID: 23862741
    18. Human IL-17A and IL-17F depend on ACT1 to mediate protective mucocutaneous immunity. PMID: 24120361
    19. Data show the contribution of TRAF3IP2 genetic variability in Systemic lupus erythematosus (SLE) susceptibility. PMID: 23836313
    20. Deletion of Act1 in NG2-positive glia results in markedly reduced experimental autoimmune encephalomyelitis in transgenic mice. PMID: 23995070
    21. our findings suggest that TRAF3IP2 variants contribute to cutaneous extarintestinal manifestations of both Crohn's disease and ulcerative colitis. PMID: 22445837
    22. In psoriatic arthritis patients the TRAF3IP2-variant p.Asp10Asn is the only susceptiblity allele with functional impact on TRAF6 binding. PMID: 22513239
    23. IL-17 receptor adaptor protein Act1/CIKS plays an evolutionarily conserved role in antiviral signaling. PMID: 23066157
    24. The findings define a new role for the IKK-related kinases in suppressing IL-17-mediated NF-kappaB activation through TRAF6-dependent Act1 phosphorylation. PMID: 22851696
    25. A CIKS mutant may promote the initiation of psoriatic inflammation PMID: 22581863
    26. Act-1 contributed to the stimulatory effect of IL-17 on RAGE production in rheumatoid arthritis PMID: 21749686
    27. Data indicate that knockdown of beta-TrCP1 and beta-TrCP2 markedly reduced IL-17-induced, phosphorylation-dependent ubiquitination and degradation of Act1. PMID: 22045853
    28. TRAF3IP2 represents a shared susceptibility for PMID: 20953188
    29. Act1 transgene functions as an important regulator of B cell activating factor (BAFF)-mediated survival of transitional B cells and their maturation into follicular and marginal zone B cells. PMID: 20543113
    30. Endogenous Act1 is recruited to the CD40 receptor in human intestinal (HT29) and cervical (HeLa) epithelial cells upon stimulation with CD40 ligand, indicating that Act1 is involved in this signaling pathway. PMID: 12089335
    31. Data indicate that the newly identified alternatively spliced Act1 is a major transcript of the molecule and that Act1 may play important roles in oncogenesis. PMID: 12163033
    32. CIKS forms homo-oligomers, interacts with NEMO/IKKgamma, and is recruited to the IKK-complex upon cell stimulation PMID: 12943667
    33. two independent and indispensable signaling pathways-1) JAK1-associated PI3K signaling and 2) Act1/TRAF6/TAK1-mediated NF-kappaB activation-are stimulated by IL-17A to regulate gene induction in human airway epithelial cells. PMID: 17982039
    34. Whereas Act1 interacts with the IL-17R through the C-terminal SEFIR domain, Act1 is recruited to CD40 and BAFFR indirectly, which is mediated by TRAF3 through the TRAF binding site in Act1[review] PMID: 18061473
    35. Act1 mediates IL-17-induced signaling pathways through its E3 ubiquitin ligase activity and TRAF6 is a critical substrate of Act1, which indicates the importance of protein ubiquitination in the IL-17-dependent inflammatory response PMID: 19825828

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  • 相关疾病：
    Psoriasis 13 (PSORS13); Candidiasis, familial, 8 (CANDF8)
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 1343

    OMIM: 607043

    KEGG: hsa:10758

    STRING: 9606.ENSP00000357750

    UniGene: Hs.561514