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  4. Recombinant Human Aldehyde dehydrogenase family 1 member A3 (ALDH1A3)

Recombinant Human Aldehyde dehydrogenase family 1 member A3 (ALDH1A3)

  • 中文名称：
    Recombinant Human Aldehyde dehydrogenase family 1 member A3(ALDH1A3)
  • 货号：
    CSB-BP001567HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Aldehyde dehydrogenase family 1 member A3(ALDH1A3)
  • 货号：
    CSB-EP001567HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Aldehyde dehydrogenase family 1 member A3(ALDH1A3)
  • 货号：
    CSB-MP001567HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:ALDH1A3 Synonyms:ALDH6
  • Uniprot No.：
  • 别名：
    Acetaldehyde dehydrogenase 6; AL1A3_HUMAN; Aldehyde dehydrogenase 1A3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; Aldh1a3; ALDH1A6; ALDH6; RALDH 3; Retinaldehyde dehydrogenase 3; V1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-512
  • 氨基酸序列
    ATANGAVEN GQPDRKPPAL PRPIRNLEVK FTKIFINNEW HESKSGKKFA TCNPSTREQI CEVEEGDKPD VDKAVEAAQV AFQRGSPWRR LDALSRGRLL HQLADLVERD RATLAALETM DTGKPFLHAF FIDLEGCIRT LRYFAGWADK IQGKTIPTDD NVVCFTRHEP IGVCGAITPW NFPLLMLVWK LAPALCCGNT MVLKPAEQTP LTALYLGSLI KEAGFPPGVV NIVPGFGPTV GAAISSHPQI NKIAFTGSTE VGKLVKEAAS RSNLKRVTLE LGGKNPCIVC ADADLDLAVE CAHQGVFFNQ GQCCTAASRV FVEEQVYSEF VRRSVEYAKK RPVGDPFDVK TEQGPQIDQK QFDKILELIE SGKKEGAKLE CGGSAMEDKG LFIKPTVFSE VTDNMRIAKE EIFGPVQPIL KFKSIEEVIK RANSTDYGLT AAVFTKNLDK ALKLASALES GTVWINCYNA LYAQAPFGGF KMSGNGRELG EYALAEYTEV KTVTIKLGDK NP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid. Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde. Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region.
  • 基因功能参考文献：
    1. We also detected increased aldehyde dehydrogenase (ALDH) activity associated with overexpression of specific ALDH isoform 1A3. Its inhibition by siRNA approach partially sensitized cells to various agents, thus linking for the first time the ALDH1A3 and chemoresistance in colorectal cancer. PMID: 30143021
    2. Data indicate that aldehyde dehydrogenase 1A3 (ALDH1A3), that is directly involved in therapy resistance of glioblastoma, is regulated by autophagy during chemotherapy. PMID: 29306018
    3. ALDH1A3 suppression could be one of PPARG tumor suppressive function. This study provides a better understanding of the role of PPARG in lung cancer. PMID: 29873276
    4. Here the s report the first crystal structure of human ALDH1A3 complexed with NAD(+) and the product all-trans retinoic acid (REA). The tetrameric ALDH1A3 folds into a three domain-based architecture highly conserved along the ALDHs family. The structural analysis revealed two different and coupled conformations for NAD(+) and REA that the s propose to represent two snapshots along the catalytic cycle. PMID: 27759097
    5. Data show that retinoic acid receptor responder 1 (RARRES1) expression is subtype-dependent and regulated by DNA methylation and the expression of aldehyde dehydrogenase 1A3 (ALDH1A3). PMID: 27286452
    6. ALDH1A3 is a poor prognostic factor for patients with intrahepatic cholangiocarcinoma who underwent hepatectomy and in those with advanced intrahepatic cholangiocarcinoma receiving chemotherapy. PMID: 27076629
    7. high transcription activities of ALDH1A2, ALDH1A3 and ALDH1L1 predicted worsen overall survival in gastric cancer patients PMID: 27015121
    8. Melanoma treatment with the novel irreversible isoform-specific ALDH1 inhibitor [4-dimethylamino-4-methyl-pent-2-ynthioic acid-S methylester] di-methyl-ampal-thio-ester (DIMATE) or depletion of ALDH1A1 and/or ALDH1A3, promoted the accumulation of apoptogenic aldehydes leading to apoptosis and tumor growth inhibition in immunocompetent, immunosuppressed and patient-derived xenograft mouse models. PMID: 28581514
    9. these findings ascribe a novel function for ALDH1A3 in an aggressive glioma stem cells phenotype via the up-regulation of tissue transglutaminase PMID: 28423611
    10. our findings define a FOXD1-ALDH1A3 pathway in controling the clonogenic and tumorigenic potential of mesenchymal glioma stem-like cells in glioblastoma tumors PMID: 27569208
    11. we looked up our single center primary prostate cancer post-operative follow-up data and suggested that the high level ALDH1A3 expression could predict the poor progression-free survival in a 158-patient cohort. We concluded that ALDH1A3, localized in luminal layer in prostate epithelium, is highly expressed in prostate cancer PMID: 28443495
    12. this report brings new information for the phenotype-genotype correlation of ALDH1A3 mutations and raises important questions, especially in terms of genetic counselling given to the patients and their families PMID: 26873617
    13. ALDH1A3 was most relevant to extracellular matrix organization and cell adhesion biological process, and the ability of tumor invasion was suppressed after ALDH1A3 knockdown in vitro. PMID: 26575197
    14. the prevalence of ALDH1A3(+)/CD44(+) tumor cells in breast cancer is significantly associated with worse prognostic factors and favors a poor prognosis PMID: 26687806
    15. Whole-exome sequencing in a South American cohort links ALDH1A3, FOXN1 and RARB/retinoic acid regulation pathways to autism spectrum disorders. PMID: 26352270
    16. we demonstrated that a specific ALDH isoform, namely ALDH1A3, is enriched in chemoresistant mesothelioma cell subpopulations PMID: 25868979
    17. ALDH1A3 induces differential RA signaling in breast cancer cells which affects the rate of breast cancer progression. PMID: 25106087
    18. ALDH1A3 is a target of miR-187 in human prostate cancer. PMID: 25969992
    19. Increased hypermethylation of ALDH1A3 is associated with Glioblastoma. PMID: 25684492
    20. Among unique genes up-regulated by Y15 in Lovo-1 and K1 resistant cells, a stem cell marker-ALDH1A3-was detected to be up-regulated >twofold. PMID: 25656374
    21. High ALDH1A3 expression is associated with non-small cell lung cancer. PMID: 24907115
    22. Mutations in ALDH1A3 represent a frequent cause of microphthalmia/anophthalmia in consanguineous families. PMID: 24777706
    23. At the transcript level, the cisplatin + DEAB-resistant cells showed upregulated mRNA expression levels for ALDH1A2, ALDH1A3 isozymes and CD44 indicating the involvement of these markers in conferring chemoresistance PMID: 24884875
    24. Nine individuals, from one inbred kindred, segregating isolated A/M with orbital cysts were homozygous for a missense mutation in the gene encoding the A3 isoform of the ALDH1A3. PMID: 23881059
    25. Novel ALDH1A3 mutations identified in the present study confirm the pivotal role of ALDH1A3 in human eye development. PMID: 24568872
    26. ALDH1A3 may be upregulated in pancreatic cancer PMID: 24053169
    27. ALDH1A3 expression determines the stem cell-like cellular states in EGFR-mutant non-small cell lung cancer; a novel mechanism of acquired resistance to erlotinib can be targeted with the natural polyphenol silibinin. PMID: 24047698
    28. Our data support the very recent and independent identification of ALDH1A3 as a disease gene in microphthalmia. PMID: 23646827
    29. Results show that ALDH1A3 loss of function causes anophthalmia and aberrant eye development in humans and in animal model systems. PMID: 23591992
    30. The effects of phenotypic and morphological alterations by DEAB on oral keratinocytes are mainly consequent to the inhibition of ALDH1A3 activity. PMID: 23250514
    31. The methylation status of Homeobox A9 (HOXA9), ISL LIM homeobox 1 (ISL1) and Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) was significantly associated with decreased gene expression levels PMID: 23436614
    32. two subtypes of GSCs, harboring distinct metabolic signaling pathways, represent intertumoral glioma heterogeneity and highlight previously unidentified roles of ALDH1A3-associated signaling that promotes aberrant proliferation of Mes HGGs and GSCs. PMID: 23650391
    33. Homozygosity for one splice-site and two missense mutations in the gene encoding the A3 isoform of the aldehyde dehydrogenase 1 (ALDH1A3) in three consanguineous families, is reported. PMID: 23312594
    34. ALDH1A3 transcripts are detected at high levels in luminal progenitor-enriched fractions obtained from short-term adherent cultures of freshly isolated normal human mammary cells. PMID: 22131125
    35. ALDH1A3 expression in patient breast tumors correlates significantly with tumor grade, metastasis, and cancer stage PMID: 21280157
    36. these results indicate that the regulation of the retinoic acid metabolism in the epidermis involves transcriptional activation of ALDH1A3, possibly representing a positive feedback loop, which enhances the effect of exogenous retinoic acid. PMID: 20709019
    37. play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. PMID: 7698756

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  • 相关疾病：
    Microphthalmia, isolated, 8 (MCOP8)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Aldehyde dehydrogenase family
  • 组织特异性：
    Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.
  • 数据库链接：

    HGNC: 409

    OMIM: 600463

    KEGG: hsa:220

    STRING: 9606.ENSP00000332256

    UniGene: Hs.459538