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  4. Recombinant Human Alpha- (1,3)-fucosyltransferase (FUT7), partial

Recombinant Human Alpha- (1,3)-fucosyltransferase (FUT7), partial

  • 中文名称：
    人FUT7重组蛋白
  • 货号：
    CSB-YP607536HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人FUT7重组蛋白
  • 货号：
    CSB-EP607536HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人FUT7重组蛋白
  • 货号：
    CSB-EP607536HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人FUT7重组蛋白
  • 货号：
    CSB-BP607536HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人FUT7重组蛋白
  • 货号：
    CSB-MP607536HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FUT7
  • Uniprot No.：
  • 别名：
    3)-fucosyltransferase; Alpha (1,3) fucosyltransferase 7; Alpha (1,3) fucosyltransferase; Alpha-(1; Fuc-TVII; Fucosyltransferase 7 (alpha (1,3) fucosyltransferase); Fucosyltransferase 7; Fucosyltransferase VII; FucT VII; FucT-VII; FUT7; FUT7_HUMAN; Galactoside 3 L fucosyltransferase; Galactoside 3-L-fucosyltransferase; Selectin ligand synthase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the N-acetyl glucosamine (GlcNAc) of a distal alpha2,3 sialylated lactosamine unit of a glycoprotein or a glycolipid-linked sialopolylactosamines chain through an alpha-1,3 glycosidic linkage and participates in the final fucosylation step in the biosynthesis of the sialyl Lewis X (sLe(x)), a carbohydrate involved in cell and matrix adhesion during leukocyte trafficking and fertilization. In vitro, also synthesizes sialyl-dimeric-Lex structures, from VIM-2 structures and both di-fucosylated and trifucosylated structures from mono-fucosylated precursors. However does not catalyze alpha 1-3 fucosylation when an internal alpha 1-3 fucosylation is present in polylactosamine chain and the fucosylation rate of the internal GlcNAc residues is reduced once fucose has been added to the distal GlcNAc. Also catalyzes the transfer of a fucose from GDP-beta-fucose to the 6-sulfated a(2,3)sialylated substrate to produce 6-sulfo sLex mediating significant L-selectin-dependent cell adhesion. Through sialyl-Lewis(x) biosynthesis, can control SELE- and SELP-mediated cell adhesion with leukocytes and allows leukocytes tethering and rolling along the endothelial tissue thereby enabling the leukocytes to accumulate at a site of inflammation. May enhance embryo implantation through sialyl Lewis X (sLeX)-mediated adhesion of embryo cells to endometrium. May affect insulin signaling by upregulating the phosphorylation and expression of some signaling molecules involved in the insulin-signaling pathway through SLe(x) which is present on the glycans of the INSRR alpha subunit.
  • 基因功能参考文献：
    1. FUT7 silencing causes inhibition of adhesion and migration of HepG2 hepatocellular carcinoma cells in vitro. PMID: 28585149
    2. FUT7 overexpression significantly promoted monocyte-endothelial adhesion, while FUT7 knockdown obviously inhibited IL-1beta-induced monocyte-endothelial adhesion PMID: 29138114
    3. The DNA demethylation within the fut7 gene controls selectin ligand expression in humans, including the inducible topographic commitment of T cells for skin and inflamed sites. PMID: 27591321
    4. This study presents the first example of a distinct regulatory mechanism involving transcriptional down-regulation of Fut7 by MAPCs that could modulate the trafficking behavior of T cells in vivo. PMID: 24176542
    5. After transfection of JAR cells with fucosyltransferase VII, the expression of FUT7 and sLeX synthesis were increased, and the percent adhesion of trophoblast cells to RL95-2 cell monolayer was significantly increased. PMID: 21197561
    6. Overexpression of alpha1,3 FucT-VII promoted the expression of CD24 and SLe X and is associated with colorectal carcinoma metastases. PMID: 20428816
    7. The Fuc-T VII promoter is transactivated by Tax in concert with CBP through a CRE-like sequence in a manner similar to that of viral CRE in HTLV-1 LTR. PMID: 12506041
    8. H-Ras mediates FucT-VII induction in Jurkat T cells via the activation of the Raf, PI3K, and a distinct, H-Ras-specific effector signaling pathway. PMID: 15262995
    9. These results suggest that the FucT-VII may be a major regulator of the biosynthesis of the sLe(x)-epitopes on T lymphoblasts. PMID: 15464988
    10. A differential functional impact of N-glycosylation on C2GnT-1 and FucT-VII and disclose that a strongly reduced FucT-VII activity retains the ability to fucosylate PSGL-1 on the core2-based binding site(s) for the three selectins. PMID: 15926890
    11. Alpha 1,3-fucosyltransferase-VII regulates the signaling molecules of the insulin receptor pathway PMID: 17229154
    12. The up regulation of alpha1,3FucT-VII mRNA and cell surface SLe(x) (alpha1,3FucT-VII product) by UV and down regulation of them by ATRA was speculated to be one of the mechanisms that alpha1,3FucT-VII decreased and increased [susceptibility to] apoptosis. PMID: 17436081
    13. Overexpression of fucosyltransferase VII (FUT7) promotes embryo adhesion and implantation. PMID: 18402946
    14. This is the first time to report that alpha1,3FucT-VII can regulate the mRNA expression of integrin. PMID: 18452157

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  • 亚细胞定位：
    Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
  • 蛋白家族：
    Glycosyltransferase 10 family
  • 组织特异性：
    Leukocytic/myeloid lineage cells.
  • 数据库链接：

    HGNC: 4018

    OMIM: 602030

    KEGG: hsa:2529

    STRING: 9606.ENSP00000318142

    UniGene: Hs.457