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  4. Recombinant Human Ankyrin repeat domain-containing protein 1 (ANKRD1)

Recombinant Human Ankyrin repeat domain-containing protein 1 (ANKRD1)

  • 货号：
    CSB-YP618002HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618002HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP618002HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618002HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618002HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ANKRD1
  • Uniprot No.：
  • 别名：
    ALRP; ANKR1_HUMAN; ANKRD 1; ANKRD1; Ankyrin repeat domain 1 (cardiac muscle); Ankyrin repeat domain containing protein 1 ; Ankyrin repeat domain-containing protein 1; bA320F15.2 ; C 193; C193; Cardiac ankyrin repeat protein; CARP; CVARP; Cytokine inducible nuclear protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; HA1A2; Liver ankyrin repeat domain 1 ; MCARP; OTTHUMP00000020084
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-319
  • 氨基酸序列
    MMVLKVEELV TGKKNGNGEA GEFLPEDFRD GEYEAAVTLE KQEDLKTLLA HPVTLGEQQW KSEKQREAEL KKKKLEQRSK LENLEDLEII IQLKKRKKYR KTKVPVVKEP EPEIITEPVD VPTFLKAALE NKLPVVEKFL SDKNNPDVCD EYKRTALHRA CLEGHLAIVE KLMEAGAQIE FRDMLESTAI HWASRGGNLD VLKLLLNKGA KISARDKLLS TALHVAVRTG HYECAEHLIA CEADLNAKDR EGDTPLHDAV RLNRYKMIRL LIMYGADLNI KNCAGKTPMD LVLHWQNGTK AIFDSLRENS YKTSRIATF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.
  • 基因功能参考文献：
    1. review of CARP, including its discovery, structure, and the role it plays in cardiac development and heart diseases [review] PMID: 27143260
    2. These data suggest that hepatitis C virus coopts ANKRD1 for its own propagation and up-regulation of ANKRD1 may contribute to hepatitis C virus-mediated liver pathogenesis. PMID: 26860204
    3. To the Ankrd1, it is not responsive to the cardiotoxic drug Doxorubicin, suggesting that different mechanisms govern their expression in cardiac cells. PMID: 25585647
    4. The association of ANKRD1 with antiapoptotic response suggests its role as myocyte survival factor during late stage heart disease, warranting further studies on ANKRD1 during end-stage heart failure. PMID: 25961010
    5. Report structure activity relationships for ANKRD1. PMID: 25125175
    6. ANKRD1 has a significant role in the regulation of apoptosis in human ovarian cancer cells PMID: 24531715
    7. Augmented CARP expression may be a common molecular event in failing hearts regardless of cardiomyopathic aetiology. PMID: 19359327
    8. Report impact of ANKRD1 mutations associated with hypertrophic cardiomyopathy on contraction parameters of engineered heart tissue. PMID: 23572067
    9. the 26S proteasome is the dominant regulator of Ankrd1/CARP degradation, and that Ankrd1/CARP half-life is significantly longer in cardiomyocytes (h) than endothelial cells (min). PMID: 22892129
    10. Ankrd1 and desmin may play important roles in airway smooth muscle cell homeostasis. PMID: 22085644
    11. CARP and its regulator calpain 3 appear to occupy a central position in the important cell fate-governing NF-kappaB pathway in skeletal muscle PMID: 20860623
    12. p53 operates as an upstream effector of Ankrd1/CARP, by up regulating the proximal ANKRD1 promoter PMID: 20599664
    13. On the basis of genetic and functional analysis of CARP mutations, ANKRD1 has been identified as a new gene associated with dilated cardiomyopathy, accounting for approximately 2% of the 231 cases studied. PMID: 19525294
    14. CD45 lateral mobility is regulated by the spectrin-ankyrin cytoskeleton of T cells PMID: 20164196
    15. Cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure. PMID: 12054667
    16. CARP expression is observed by in situ hybridization in endothelial cells lining human atherosclerotic plaques, whereas lesion macrophages are devoid of CARP; it is induced by activin A in cultured intimal smooth muscle cells. PMID: 12524226
    17. endogenous ANKRD1 and calsequestrin are co-enriched in piglet cardiac Purkinje cells PMID: 15698842
    18. CARP interacts with itself and desmin. CARP, ankrd2, and DARP contain potential coiled-coil dimerization motifs within their unique aminoterminal domains that mediate the formation of homo-dimers. PMID: 16450059
    19. CARP was expressed in renal podocytes at a high level in 10 of 13 cases of crescentic glomerulonephritis, 7 of 19 cases of diabetic nephropathy, and 12 of 20 cases of lupus nephritis. PMID: 17239933
    20. cloned both translocation breakpoints and mapped the ANKRD1 gene, encoding a cardiac transcriptional regulator, 130 kb proximally to the breakpoint on chromosome 10, in total anomalous pulmonary venous return, a congenital heart defect PMID: 18273862
    21. Our results indicate that CARP is a sensitive and specific marker for rhabdomyosarcoma and that it will be useful for the differential diagnosis of rhabdomyosarcoma. PMID: 18656235
    22. We suggest that CDH1 cytoplasmic immunolocalization as a result of increased IGF-II levels identifies those nonmuscle invasive presentations most likely to recur PMID: 18980987
    23. Data show that ANKRD1 is a short-lived protein whose levels are tightly regulated by the 26S proteasome. PMID: 19589340
    24. ANKRD1 is a novel DCM gene, with mutations present in 1.9% of DCM patients. The ANKRD1 mutations may cause DCM PMID: 19608030
    25. CARP abnormalities may be involved in the pathogenesis of HCM. PMID: 19608031

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  • 相关疾病：
    Total anomalous pulmonary venous return (TAPVR)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.
  • 数据库链接：

    HGNC: 15819

    OMIM: 106700

    KEGG: hsa:27063

    STRING: 9606.ENSP00000360762

    UniGene: Hs.448589