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  4. Recombinant Human Apoptotic protease-activating factor 1 (APAF1), partial

Recombinant Human Apoptotic protease-activating factor 1 (APAF1), partial

  • 货号：
    CSB-YP001885HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    37.4kDa
  • 表达区域：
    104-415aa
  • 氨基酸序列
    SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • 基因功能参考文献：
    1. APAF1 mutations cause recurrent folate-resistant neural tube defects. PMID: 29358613
    2. Study suggests a potential underlying molecular mechanism of apoptosis inhibition via APAF1 downregulation in human neuroblastoma BE(2)C cells with miRNA36133p overexpression. PMID: 30066861
    3. Down-regulation of Apaf-1 protein and the overexpression of Cyclin D1 and AQP-5 proteins possibly contribute to an aggressive serous ovarian carcinoma with a high risk of recurrence and poor response to the first-line chemotherapy. PMID: 30072015
    4. Results suggest that low levels of Apaf-1 as an adaptor protein might be considered as a possible regulatory barrier by which differentiating cells control cell death upon rise in ROS production and cytochrome c release from mitochondria. PMID: 29395479
    5. APAF-1 was found to be reduced in non-small-cell lung cancer tissue samples along with high expression of miR-484. PMID: 28982084
    6. miR-300 regulates the cellular sensitivity to ionizing radiation through targeting p53 and apaf1 in lung cancer cells. PMID: 28895780
    7. we sought to investigate mechanisms mediated by Hsp70 acetylation in relation to apoptotic and autophagic programmed cell death. Upon stress-induced apoptosis, Hsp70 acetylation inhibits apoptotic cell death, mediated by Hsp70 association with apoptotic protease-activating factor (Apaf)-1 and apoptosis-inducing factor (AIF), key modulators of caspase-dependent and -independent apoptotic pathways, respectively PMID: 28627586
    8. Knockdown of microRNA-27a increased the expression level of Apaf-1, enhancing the formation of Apaf-1-caspase-9 complex and subsequently promoting the TRAIL-induced apoptosis in colorectal cancer stem cells. Findings suggested that knockdown of microRNA-27a in colorectal cancer stem cells by the specific antioligonucleotides was potential to reverse the chemoresistance to TRAIL. PMID: 28423356
    9. Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID: 28143931
    10. s found significant up-regulation of miR-221 and significant down-regulation of Apaf-1 expression in LSCC tissues compared to normal nearby laryngeal tissues. Significant associations between up-regulated miR-221 and down-regulated Apaf-1 expressions and clinical stage and lymph node (LN) metastasis were found. PMID: 28453462
    11. A significant correlation between changes in the levels of expression and methylation was detected for the three apoptosis-regulatory genes (APAF1, DAPK1, and BCL2). The results suggest that methylation play an important role in the regulation of the apoptosis system genes in breast cancer. PMID: 28429233
    12. Primary cells derived from patients with diffuse large B cell lymphomas show membrane raft sequestration of the apoptosome adaptor protein, Apaf-1, which may mediate drug resistance. PMID: 27863378
    13. Loss of APAF-1 expression is associated with early recurrence in stage I-III colorectal cancer, suggesting that APAF-1 may have clinical value as a predictive marker of early recurrence. PMID: 27480415
    14. the markers Ets-1 and APAF-1 relative to p53, Ki-67 and PTEN expression in colon adenomas/polyps, were investigated. PMID: 26743285
    15. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD PMID: 26179084
    16. miR21 is transferred from cancer-associated adipocytes (CAAs) and fibroblasts (CAFs) to the cancer cells, where it suppresses ovarian cancer apoptosis and confers chemoresistance by binding to its direct novel target, APAF1. PMID: 27021436
    17. HDAC inhibitors can induce p53 acetylation at lysine 120, which in turn enhances mitochondrion-mediated apoptosis through transcriptional up-regulation of Apaf-1. PMID: 26851285
    18. Renal APAF1 expression is increased in diabetes and diabetic nephropathy. PMID: 27141571
    19. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
    20. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation. PMID: 26740177
    21. The auto-assembly of truncated Apaf1 molecules disappeared after several hours without any enzyme activation. PMID: 25895636
    22. cytochrome c and the WD domains of Apaf-1 interact, with bifurcated salt bridges involved in apoptosome assembly PMID: 26014357
    23. NF-kappaB response element, located close to the p53RE#1, mediates APAF1 transcriptional repression by affecting interaction between KAISO and p53 PMID: 26183023
    24. This study suggested that miR-23a, acting as an oncogenic regulator by directly targeting APAF1 in pancreatic cancer, is a useful potential biomarker in diagnosis and treatment of pancreatic cancer. PMID: 25701323
    25. Our results indicate that the APAF1, BAX, and FLASH genes not only harbor frameshift mutations but also demonstrate mutational ITH, which together might play a role in the tumorigenesis of CRC with MSI-H by affecting the apoptosis of cancer cells. PMID: 25599959
    26. a role for Apaf1 at the mitochondria PMID: 24416260
    27. APAF-1 plays an important role in iron-induced erythroid apoptosis increase in myelodysplastic syndrome PMID: 24142530
    28. The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID: 24424093
    29. Apoptosis of HGC-27 cells induced by oridonin may be associated with differential expression of Apaf-1, caspase-3 and cytochrome c, which are highly dependent upon the mitochondrial pathway PMID: 23326121
    30. Amiloride modulates glioblastoma multiforme cell radiosensitivity involving the Akt phosphorylation and the alternative splicing of APAF1. PMID: 23822711
    31. Apaf-1 conformational changes during apoptosome assembly. PMID: 23521171
    32. Data indicate that the formation of cytochrome c-Apaf-1 apoptosome and the presence of Smac are absolutely required for PSAP-induced apoptosis. PMID: 23207240
    33. Both the silencing of miR-155 and the overexpression Apaf-1 greatly increased the sensitivity of A549 cells to cisplatin. PMID: 22996741
    34. The 5' untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5' end-dependent scanning mechanism. PMID: 23085065
    35. Curcumin treatment of cancer cells led to Apaf-1 upregulation both at the protein and mRNA levels. Deficiency of Apaf-1 inhibits caspase activation and cell death. PMID: 22101335
    36. APAF-1, DAPK-1 and SPARC may have a role in progression and higher tumor stage of renal cell carcinoma in North Indian population PMID: 21922274
    37. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
    38. These results suggest that, in addition to the classical cytochrome c/Apaf-1-dependent pathway of caspase-9 activation, staurosporine can induce caspase-9 activation and apoptosis independently of the apoptosome. PMID: 21659556
    39. Results show a significant inverse correlation between GLUT1 expression and Apaf-1 expression in colorectal adenocarcinomas, and patients with GLUT1 expression demonstrate poor overall survival. PMID: 21528061
    40. in the absence of nucleotide such as ATP, direct association of procaspase-9 with Apaf-1 leads to defective molecular timer, and thus, inhibits apoptosome-mediated caspase activation PMID: 21297999
    41. Expression of Apaf-1 was up-regulated in MCF-7 cells treated with 5-Aza-CdR. PMID: 19662370
    42. Hypermethylation of APAF1 gene is associated with neuroblastoma. PMID: 21104989
    43. higher expression of APAF-1 is related to an undifferentiated state in the testicular germ cell tumor (TGCT) pathway. PMID: 20977544
    44. APAF-1 expression was significantly higher in low-risk, compared to high-risk myelodysplastic syndrome, according to IPSS (P < 0.0001), FAB (P = 0.0265), and cytogenetic risk (P = 0.0134). PMID: 20345447
    45. results show that the APAF1 variants associated with risk for MDD in the Utah pedigrees are very rare in Northern European and European-American populations PMID: 19455599
    46. Studies suggest that the apoptosome contains a 1:1 Apaf-1:caspase-9 stoichiometry. PMID: 19809088
    47. A drug targeting Apaf-1 allows protection from apoptosis as well as regeneration in the course of inflammation-induced tissue injury PMID: 19675677
    48. Apaf1 WD40 repeat domains form propellar-like structures that are important for procaspase 9 binding to the CARD domain. PMID: 11864614
    49. lack of role in caspase-9 activation in Sendai virus-infected cells PMID: 12021264
    50. APAF-1 is under transcriptional regulation of E2F-1 and initiates a caspase cascade PMID: 12149244

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  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • 数据库链接：

    HGNC: 576

    OMIM: 602233

    KEGG: hsa:317

    STRING: 9606.ENSP00000448165

    UniGene: Hs.552567