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  4. Recombinant Human Bone marrow stromal antigen 2 (BST2), partial

Recombinant Human Bone marrow stromal antigen 2 (BST2), partial

  • 货号：
    CSB-YP606010HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP606010HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP606010HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP606010HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP606010HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BST2
  • Uniprot No.：
  • 别名：
    Bone marrow stromal antigen 2; Bone marrow stromal cell antigen 2; Bone marrow stromal cell antigen; BST 2; BST-2; BST2; BST2_HUMAN; CD 317; CD317; CD317 antigen; HM1.24 antigen; NPC A 7; Tetherin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV. Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities. Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu. Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2.
  • 基因功能参考文献：
    1. The primary role of the S(52,56) residues of Vpu in antagonism of CD4, GaLV Env, and BST-2/tetherin is to recruit the SCF/betaTrCP ubiquitin ligase. PMID: 30347660
    2. Studied role of bone marrow stromal cell antigen 2 (BST2) in gastric cancer (GC); results show BST2 is overexpressed in GC tissues and BST2 silencing inhibits cell proliferation and migration, partly by regulating NF-kappaB signaling. PMID: 29774441
    3. The results establish tetherin as a key effector of the intrinsic immune defense against HIV-1, and they demonstrate that Vpu-mediated tetherin antagonism is critical for efficient viral spread during the initial phase of HIV-1 replication. PMID: 29324226
    4. findings support the notion that NAbs can induce ADCC. They highlight that while BST2 antagonism by HIV promotes ADCC evasion, strategies aimed at restoring BST2 restriction could improve anti-HIV responses and potentially provide a means to eliminate reactivated cells in latent reservoirs. PMID: 27853288
    5. BST2 likely mediates platinum resistance in nasopharyngeal cancer via NF-kappaB signaling PMID: 28617432
    6. These data collectively suggest that the human parainfluenza virus type 2 V protein inhibits tetherin expression induced by several external stimuli. PMID: 28455649
    7. These results suggest that tetherin antagonism by V proteins is common among the genus Rubulavirus. PMID: 28466381
    8. High BST2 expression is associated with Esophageal, Gastric, or Colorectal Cancer. PMID: 26832883
    9. Two additional regulators of BST2 constitutive ubiquitylation and sorting to the lysosomes: the E3 ubiquitin ligases NEDD4 and MARCH8, are reported. PMID: 28320822
    10. Coimmunoprecipitation studies indicated that non-glycosylated tetherin is stabilized through the formation of a ternary SGTA/Vpu/tetherin complex. Although the results do not provide support for a physiological function of SGTA in HIV-1 replication, they demonstrate that SGTA overexpression regulates tetherin expression and stability, thus providing insights into the function of SGTA in endoplasmic reticulum translocation PMID: 27103333
    11. Importantly, the s demonstrate for the first time that the HIV-2 Env induces NF-kappaB activation in HEKappa293T cells. Furthermore, the anti-BST-2 activity of the HIV-2 Env is not sufficient to completely inhibit NF-kappaB activity. PMID: 29028477
    12. Data revealed that under a nutrient deficient condition, CD317 functions as an anti-apoptotic factor through AIF-mediated caspase and autophagy-independent manner. PMID: 27444183
    13. findings lead us to believe that BISPR and BST2 may regulate egress of HEV virions into bile in vivo. This system may also be used to scale up virus production in vitro PMID: 29091957
    14. a non-canonical autophagy pathway reminiscent of LC3-associated phagocytosis contributes to Vpu counteraction of BST2 restriction. PMID: 27880899
    15. Ebola virus GP1,2, the Ebola virus matrix protein VP40, and BST2 are at least additive with respect to the induction of NF-kappaB activity. PMID: 28878074
    16. Studied the mechanism of viral budding and tethering mediated by human BST-2/tetherin using a model of BST-2 embedded in a membrane and used steered molecular dynamics to simulate the transition from the host cell membrane associated form to the cell-virus membrane bridging form. PMID: 28779494
    17. Disruption of BST-2 dimerization offers a potential therapeutic approach for breast cancer. PMID: 28300825
    18. identified WDR81 as a novel gene required for tetherin trafficking and degradation in both the presence and absence of Vpu PMID: 27126989
    19. Among 32 HIV-2 ROD Env mutants tested, the s demonstrated that the asparagine residue at position 659 located in the gp36 ectodomain is mandatory to exert the anti-tetherin function. PMID: 27754450
    20. Despite the influence of rs919266 and rs9576 on BST2 expression being still undetermined, a preventive role by BST2 polymorphisms was found during HIV-1 infection. PMID: 26885809
    21. The s demonstrated that BST2 restricts the release of Japanese encephalitis virus whose budding occurs at the endoplasmic reticulum-Golgi intermediate compartment, and in turn, Japanese encephalitis virus downregulates BST2 expression via envelope protein E. PMID: 28710958
    22. These results suggest that BST2 plays an important role in the progression of renal cell carcinoma (RCC), and, because BST2 is expressed on the cell membrane, BST2 is a good therapeutic target for RCC. PMID: 28551621
    23. findings show that BST-2 upregulation by IFN-beta and interleukin-27 (IL-27) also increases the surface expression of Env and thus boosts the ability of CD4mc to sensitize HIV-1-infected cells to ADCC by sera from HIV-1-infected individuals. PMID: 28331088
    24. study found that tetherin expression on hematopoietic cells resulted in the specific reduction of Moloney murine leukemia virus cell-free plasma viremia but not the number of infected hematopoietic cells PMID: 28381565
    25. The s show that orthobunyaviruses viruses with human tropism (Oropouche virus and La Crosse virus) are restricted by sheep BST-2 but not by the human orthologue, while viruses with ruminant tropism (Schmallenberg virus and others) are restricted by human BST-2 but not by the sheep orthologue. PMID: 28628828
    26. Thus, efficient human immunodeficiency virus type 1 release from infected cells seems to play an important role in the spread of the virus in the human population and requires a fully functional Vpu protein that counteracts human tetherin. PMID: 27531907
    27. BST-2 restricts influenza A virus release and is countered by the viral M2 protein. PMID: 28087685
    28. IL8 might play an important role in the enhancement of BST2 and be involved in HBsAg eradication. PMID: 28363866
    29. Antagonism of BST-2 is a conserved function of the env glycoprotein of primary HIV-2 isolates. PMID: 27681141
    30. These data present the first example of an HIV-1 group O Vpu that efficiently antagonizes human tetherin and suggest that counteraction by O-Nefs may be suboptimal. PMID: 28077643
    31. This is in contrast to the pandemic HIV-1 group M-specified BST2 countermeasure Vpu. PMID: 27581991
    32. The s show that while the conserved transmembrane-proximal Vpu hinge region residues have no intrinsic activity on the cellular distribution of Vpu in the absence of BST2, they regulate the ability of Vpu to bind to BST2 and, consequently, govern both BST2-dependent trafficking properties of the protein as well as its co-localization with BST2. PMID: 28288652
    33. In this study, we addressed this question by analysing sLex expression together with two glycoproteins (BST-2 and LGALS3BP).Concomitant high expression of BST-2 with sLex defined a sub-group of patients with ER-negative tumours displaying higher risks of liver and brain metastasis and a 3-fold decreased survival rate PMID: 27176937
    34. Studies demonstrate that the interaction Bst-2 and MT1-MMP, actually happens and the cytoplasmic tails, both the N-terminal domain of Bst-2 and the C-terminal domain of MT1-MMP, play crucial roles in the interaction. The interaction between Bst-2 and MT1-MMP is important in MT1-MMP regulating the tetherin activity of Bst-2 and also in Bst-2 regulating the activity of the MT1-MP/proMMP2/MMP2 pathway. PMID: 27240342
    35. BST-2 and HIV-2 Env proteins interact through their ectodomain but residues involved are not clearly defined. In this study, we demonstrated the importance of the asparagine residue at position 659 in the HIV-2 gp36 ectodomain for the anti-tetherin function. This study also demonstrated the involvement of the HIV-2 Env cytoplasmic tail in this antagonistic role. PMID: 27754450
    36. results herein demonstrated that IMB-LA could specifically inhibit the degradation of BST-2 induced by Vpu, and impair HIV-1 replication in a BST-2 dependent manner PMID: 26669976
    37. BST-2 inhibits the the release of Hepatitis B virus particles. [review] PMID: 27396167
    38. Combining these results suggests an important role for the Ebola virus glycoprotein glycan cap and membrane spanning domain in tetherin antagonism. PMID: 26516900
    39. BST2 showed significantly elevated plasma levels and overexpression of BST2 in CRC tissues that correlated with poor survival of colorectal cancer patients. PMID: 26494939
    40. Human parainfluenza virus type 2 V protein antagonizes tetherin by binding it and reducing its presence at the cell surface. PMID: 26675672
    41. studies suggest that Vpu hijacks the FLNa function in the modulation of tetherin to neutralize the antiviral factor tetherin. PMID: 26742839
    42. Using viral tethering, amino acid level insights into the function of BST-2 were identified. PMID: 26789136
    43. BST-2 restricts Hepatitis B virus production at intracellular multivesicular bodies. PMID: 26119070
    44. results shed light on the interaction between HIV-2 Env and tetherin, suggesting a physical interaction that maps to the ectodomains of both proteins and indicating a strong selection pressure to maintain an anti-tetherin activity in the HIV-2 Env PMID: 26248668
    45. This study shows that through a targeted regulation of surface BST2, Vpu promotes HIV-1 release and limits plasmacytoid dendritic cells antiviral responses upon sensing of infected cells PMID: 26172439
    46. The early evolution of antiviral activity together with the high topology conservation but low sequence homology suggests that restriction of virus release is the primary function of tetherin. PMID: 26401043
    47. Our results suggest that CD317 expression might be of prognostic significance for B-cell chronic lymphocytic leukemia PMID: 25973046
    48. These findings support BST2 as a genetic susceptibility factor for HIV-1 acquisition by identifying a novel single nucleotide polymorphism association for rs13189798. PMID: 25985399
    49. Vpu-mediated enhancement of HIV-1 release is uncoupled from Vpu-mediated tetherin degradation. PMID: 25360760
    50. Our study suggests that the DNA methylation pattern and expression of BST-2 may play a role in disease pathogenesis and could serve as a biomarker for the diagnosis of breast cancer. PMID: 25860442

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  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network. Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft. Cytoplasm. Apical cell membrane.
  • 蛋白家族：
    Tetherin family
  • 组织特异性：
    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly exp
  • 数据库链接：

    HGNC: 1119

    OMIM: 600534

    KEGG: hsa:684

    STRING: 9606.ENSP00000252593

    UniGene: Hs.118110