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  4. Recombinant Human CASP8 and FADD-like apoptosis regulator (CFLAR), partial

Recombinant Human CASP8 and FADD-like apoptosis regulator (CFLAR), partial

  • 货号：
    CSB-YP005282HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005282HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005282HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005282HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005282HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; cellular flice inhibitory protein; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; OTTHUMP00000163715; OTTHUMP00000206475; OTTHUMP00000206476; OTTHUMP00000206478; OTTHUMP00000206479; OTTHUMP00000206480; OTTHUMP00000206482; OTTHUMP00000207360; Usurpin; Usurpin beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 表达区域：
    1-376aa
  • 氨基酸序列
    MSAEVIHQVE EALDTDEKEM LLFLCRDVAI DVVPPNVRDL LDILRERGKL SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSLKDPSNN FRLHNGRSKE QRLKEQLGAQ QEPVKKSIQE SEAFLPQSIP EERYKMKSKP LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG KPKMFFIQNY VVSEGQLEDS SLLEVD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • 基因功能参考文献：
    1. cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID: 29222334
    2. High c-FLIP expression is associated with resistance to sorafenib via reducing ER stressrelated autophagy in hepatocellular carcinoma. PMID: 30066934
    3. The knockdown of endogenous c-FLIP revealed that this protein regulates hepatitis B virus replication through two different mechanisms. (i) c-FLIP interacts with HBx and protects it from ubiquitin-dependent degradation. (ii) c-FLIP regulates the expression or stability of hepatocyte nuclear factors (HNFs), which have critical roles in HBV transcription and maintenance of hepatocytes. PMID: 29875248
    4. Nogo-B was shown to play an important negative role in apoptotic signaling through its interaction with c-FLIP in colorectal cancer cells. PMID: 29684585
    5. Compared to normal tissues, hepatocellular carcinoma tissues had lower miR-20 and higher CFLAR expression. PMID: 28537677
    6. Investigations of underlying molecular mechanisms of TNFR1 signaling showed that PDF affects TNFR1 signaling at the proapoptotic signaling pathway by upregulation of IkappaBalpha and downregulation of cFLIPL. PMID: 28096440
    7. c-FLIP expression was significantly decreased in organs of septic rats compared with control rats, and that c-FLIP overexpression protected HUVECs from LPS+CHX-induced apoptosis in vitro. PMID: 28498469
    8. case-control study, including 600 hepatocellular carcinoma (HCC) and 600 HBsAg positive controls without HCC, was conducted to assess the relationship between 11 tagging SNPs in CASP8, CASP10 and CFLAR and HBV-related HCC risk .These results suggest that the CASP8 -652 6N ins/del polymorphism may play a protective role in the development, progression, and survival of HBV-related HCC among the Chinese Han population. PMID: 28643196
    9. Cordycepin induces apoptosis through autophagy-mediated downregulation of c-FLIPL in human non-small cell lung carcinoma cells. PMID: 28035061
    10. High c-FLIP expression is associated with lung cancer. PMID: 26992204
    11. miRNA-708 acts as a tumor suppressor because it negatively regulates the anti-apoptotic protein c-FLIPL and regulates the sensitivity of renal cancer cells to various apoptotic stimuli. PMID: 27092874
    12. The (fli:GFP) Casper zebrafish embryo can be used as an efficient animal model to study metastatic behavior of human CM cells and warrants further testing of drug efficacy to aid care of CM patients. PMID: 29204645
    13. Inhibition of BET bromodomain-dependent XIAP and FLIP expression sensitizes KRAS-mutated non-small cell lung cancer to pro-apoptotic agents. PMID: 27607580
    14. the c-FLIP and NOXA/Mcl-1 axis participated in the synergistic effect of pemetrexed plus cisplatin in human choroidal melanoma cells PMID: 28863158
    15. The strongest interaction result in relation to overall breast cancer risk was found between CFLAR-rs7558475 and current smoking (ORint = 0.77, 95% CI: 0.67-0.88, pint = 1.8 x 10(-4) ). The interaction with the strongest statistical evidence was found between 5q14-rs7707921 and alcohol consumption (ORint =1.36, 95% CI: 1.16-1.59, pint = 1.9 x 10(-5) ) in relation to ER- disease risk PMID: 28670784
    16. Data in this study show that cFLIPL inhibits IFN regulatory factor 3 (IRF3), a transcription factor central for IFN-beta and IFN-stimulated gene expression. PMID: 27342840
    17. CFLAR levels were substantially decreased in the livers of subjects with NAFLD and NASH, as compared to that in nonsteatotic controls. PMID: 28218919
    18. CFLAR role in the necroptosis in fibroblasts PMID: 27721066
    19. Our results postulate that thymoquinone induces apoptosis through downregulating c-FLIP and Bcl-2 which can be utilized as a chemotherapeutic agent to treat renal carcinoma. PMID: 27573448
    20. Data show that heterogeneous nuclear ribonucleoprotein K (hnRNPK) stabilized of cellular FLICE-inhibitory protein (c-FLIP) protein through inhibition of glycogen synthase kinase 3 beta (GSK3beta) Ser9 phosphorylation during the TNF-related aoptosis-inducing ligand (TRAIL)-induced apoptosis. PMID: 26972480
    21. cFLIP long form shRNA serves a specific inhibitory role in cellular proliferation through inducing the activation of the JNK pathway in A875 cells. PMID: 26847085
    22. Study demonstrated the function of FLIPL in facilitating hepatocellular carcinoma cells (HCC) aerobic glycolysis by modulating SGLT1-mediated glucose uptake and FLIPL expression level was positively correlated with SGLT1 expression level in patients with HCC. PMID: 27178057
    23. Data show that suppression of autophagy effectively attenuated cytotoxicity induced by Chal-24 and TRAIL combination, which was associated with attenuation of c-FLIP(L) protein and cellular inhibitor of apoptosis proteins (c-IAPs) degradation. PMID: 26448608
    24. neither c-FLIP nor procaspase-8 prodomain terminates the DED chain, but rather the dissociation/association rates of procaspase-8 define the stability of the chain and thereby its length. PMID: 26494467
    25. High FLIP expression is associated with Pancreatic cancer. PMID: 26716649
    26. A dual role for cFLIP in the maintenance of breast cancer stem cell viability, dependent upon its subcellular distribution. PMID: 26667821
    27. This conservation of IKK activation among the cFLIP family using different mechanisms suggests that the mechanism plays a critical role in their function. PMID: 26865630
    28. procaspase-8:c-FLIPL exhibits localized enzymatic activity and is preferentially an activator, promoting DED-mediated procaspase-8 oligomer assembly, whereas procaspase-8:c-FLIPS lacks activity and potently blocks procaspase-8 activation PMID: 26990987
    29. Reduced miR-512 and the elevated expression of its targets cFLIP and MCL1 localize to neurons with hyperphosphorylated Tau protein in Alzheimer disease. PMID: 26258756
    30. CDDO-Me rapidly reduced the protein levels of c-FLIPL and overexpression of c-FLIPL blocked CDDO-Me-induced cell death, but not vacuolation PMID: 26053096
    31. Identification and Characterization of the Interaction Site between cFLIPL and Calmodulin. PMID: 26529318
    32. Upregulation of hTERT+ in human lung epithelial cells and subsequent downregulation of FLIP by shFLIP-RNA annulled hTERT-mediated resistance to apoptosis. PMID: 25951185
    33. Activation of AKR1C1/ERbeta induces apoptosis by downregulation of c-FLIP in prostate cancer cells. PMID: 25816367
    34. The knockdown of c-FLIPL led to enhanced Th1 differentiation and elevated IL-4 production by Th2 cells, whereas the knockdown of c-FLIPS diminished GATA3 expression and IL-4 production. PMID: 25019384
    35. A complete inhibition of PS1-induced apoptosis was achieved by knockdown of PS1-associated protein (PSAP), a mitochondrial proapoptotic protein that forms a complex with Bax upon induction of apoptosis, in the presence of gamma-secretase inhibitor. PMID: 26025363
    36. Data show that decreased type I interferon gene expression in T cell subsets of human CASP8 and FADD-like apoptosis regulating protein c-FLIPS transgenic female mice. PMID: 24816846
    37. Increased c-FLIP expression is associated with cutaneous T-cell lymphoma. PMID: 25578476
    38. The four structure-based mutants (E80A, L84A, K169A and Y171A) of c-FLIP death effector domains disturb the interaction with FADD death effector domain, and that these mutations lower the stability of the c-FLIP death effector domain. PMID: 24355299
    39. Transfection of the c-FLIPp43 expression vector may induce the protein expression of NF-kappaBp65 and promote the activation of the NF-kappaB signaling pathway in the A375 melanoma cell line. PMID: 25017325
    40. Over-expression of FLIP-L is associated with breast cancer. PMID: 25230899
    41. cytoplasmic c-FLIPL/s was an independent prognostic parameters for progression-free survival in locally advanced rectal cancer PMID: 24711168
    42. c-FLIP negativity identifies 9% of patients with a highly aggressive disease course (P = 0.0001) and thus is a valuable prognostic biomarker in pancreatic cancer. PMID: 24005232
    43. cFLIP upregulated the expression of viral restriction factors. PMID: 23696271
    44. ERK controls epithelial cell death receptor signalling and c-FLIP in ulcerative colitis PMID: 23371318
    45. The knockout of c-FLIP and Hsp27 genes together enhances apoptosis even under 1 mu M, rather than low concentration, of doxazosin in PC-3 cells. PMID: 23853530
    46. An increased expression of c-FLIP may be an important factor in the progression of cervical cancer PMID: 23518915
    47. findings provide the first evidence showing that mTORC2 stabilizes FLIP(S), hence connecting mTORC2 signaling to the regulation of death receptor-mediated apoptosis PMID: 23319802
    48. novel ROS-dependent post-translational modifications of the c-FLIP protein that regulate its stability, thus impacting sensitivity of cancer cells to TRAIL. PMID: 23519470
    49. review of molecular mechanisms that control c-FLIP expression and current research into inhibitors of the protein [review] PMID: 21071136
    50. review of the structural and functional biology of c-FLIP with direct relevance to carcinogenesis [review] PMID: 22781394

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  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • 数据库链接：

    HGNC: 1876

    OMIM: 603599

    KEGG: hsa:8837

    STRING: 9606.ENSP00000312455

    UniGene: Hs.390736